Dissemination of antibiotic resistance genes and human pathogenic bacteria from a pig feedlot to the surrounding stream and agricultural soils

JOURNAL OF HAZARDOUS MATERIALS

卷:357

Article

Fang, Hua¹  Han, Lingxi¹  Zhang, Houpu¹  Long, Zhengnan¹  Cai, Lin²  Yu, Yunlong¹ 

[1] Zhejiang Univ, Coll Agr & Biotechnol, Inst Pesticide & Environm Toxicol, Hangzhou 310058, Zhejiang, Peoples R China

[2] Hong Kong Univ Sci & Technol, Div Life Sci, Hong Kong, Peoples R China

关键词: Antibiotic;    Manure;    Antibiotic resistance gene;    Human pathogenic bacteria;    Dissemination;   

DOI  :  10.1016/j.jhazmat.2018.05.066

来源: Elsevier

【 摘 要 】

The dissemination of antibiotic resistance genes (ARGs), human pathogenic bacteria (HPB), and antibiotic-resistant HPB (ARHPB) from animal feedlot to nearby environment poses a potentially high risk to environmental ecology and public health. Here, a metagenomic analysis was employed to explore the dissemination of ARGs, HPB, and ARHPB from a pig feedlot to surrounding stream and agricultural soils. In total, not detectable (ND) 1,628.4 mu g/kg of antibiotic residues, 18 types of ARGs, 48 HPB species, and 216 ARB isolates were detected in all samples. Antibiotic residues from pig feedlot mainly migrated into stream sediments and greenhouse soil. The dominant ARGs and HPB species from pig feedlot spread into stream sediments (tetracycline resistance genes, Clostridium difficile, and Mycobacterium tuberculosis), stream water (multidrug resistance (MDR) genes, Shigella flexneri, and Bordetella pertussis), and greenhouse soil (MDR genes, Bacillus andvacis, and Brucella melitensis). It is concerning that 54.4% of 216 ARB isolates from all samples were potential ARHPB species, and genome sequencing and functional annotation of 4 MDR HPB isolates showed 9 ARG types. Our findings revealed the potential migration and dissemination of antibiotic residues, ARGs, HPB, and ARHPB from pig feedlot to surrounding stream and agricultural soils via pig sewage discharge and manure fertilization.

【 授权许可】

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