Bio-based and cost effective method for phenolic compounds removal using cross-linked enzyme aggregates

JOURNAL OF HAZARDOUS MATERIALS

卷:403

Article

Sellami, Kheireddine^1,2  Couvert, Annabelle²  Nasrallah, Noureddine¹  Maachi, Rachida¹  Tandjaoui, Nassima³  Abouseoud, Mahmoud^1,4  Amrane, Abdeltif² 

[1] Univ Sci & Technol Houari Boumediene, Fac Genie Mecan & Genie Proc, Lab Genie React, Bab Ezzouar 16111, Alger, Algeria

[2] Univ Rennes, ISCR Inst Sci Chim Rennes UMR 6226, CNRS, Ecole Natl Super Chim Rennes, F-35000 Rennes, France

[3] Univ Ibn Khaldoun Tiaret, Dept Chim, Tiaret 14000, Algeria

[4] Univ Yahia Fares Medea, Lab Biomat & Phenomenes Transport, Fac Sci & Technol, RN1, Medea 26000, Algeria

关键词: Peroxidase immobilization;    Phenols;    CLEAs;    Enzymatic transformation;    Green process;   

DOI  :  10.1016/j.jhazmat.2020.124021

来源: Elsevier

【 摘 要 】

This work aimed at presenting a green method using a new source of peroxidase isolated from Raphanus sativus var. niger (RSVNP) in immobilized form, for the treatment of wastewater. To ensure stability and enzymatic activity in the biodegradation process, RSVNP was immobilized as a cross-linked enzyme aggregate (CLEAs). With more than 29% of recovered activity and 85% aggregation yield, acetone was selected as the best precipitating agent. The formed protein aggregates required 2% (v/v) of glutaraldehyde (GA) concentration and a ratio of 9:1 (v/v) enzyme (E) amount to cross-linker (E/GA). Compared to the free enzyme, RSVNP-CLEAs were found more chemically and thermally stable and exhibited good storage stability for more than 8 weeks. In addition, RSVNP-CLEAs were evaluated for their ability to remove phenol and p-cresol from aqueous solution by varying several operating conditions. A maximal yield (98%) of p-cresol conversion was recorded after 40 min; while 92% of phenol was degraded after 1 h duration time. The reusability of RSVNP-CLEAs was tested, displaying 71% degradation of phenol in the third batch carried out and more than 54% was achieved for p-cresol after four successive reuses in the presence of hydrogen peroxide at 2 mM concentration.

【 授权许可】

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