Complete removal of arsenic and zinc from a heavily contaminated acid mine drainage via an indigenous SRB consortium

JOURNAL OF HAZARDOUS MATERIALS

卷:321

Article

Le Pape, Pierre¹  Battaglia-Brunet, Fabienne²  Parmentier, Marc²  Joulian, Catherine²  Gassaud, Cindy²  Fernandez-Rojo, Lidia³  Guigner, Jean-Michel¹  Ikogou, Maya¹  Stetten, Lucie¹  Olivi, Luca⁴  Casiot, Corinne³  Morin, Guillaume¹ 

[1] UPMC Univ Paris VI, UMR IRD 206, Sorbonne Univ, IMPMC, 4 Pl Jussieu, F-75252 Paris 05, France

[2] French Geol Survey BRGM, 3 Ave Claude Guillemin,BP 36009, F-45060 Orleans 2, France

[3] HydroSci Montpellier, UMR CNRS IRD UM 5569, CC57, 163 Rue Auguste Broussonet, F-34090 Montpellier, France

[4] Sincrotrone Trieste ELETTRA, I-34012 Trieste, Italy

关键词: Arsenic;    Bioremediation;    Sulfate-reducing bacteria;    Biogenic sulfides;    Acid mine drainage;   

DOI  :  10.1016/j.jhazmat.2016.09.060

来源: Elsevier

【 摘 要 】

Acid mine drainages (AMD) are major sources of pollution to the environment. Passive bio-remediation technologies involving sulfate-reducing bacteria (SRB) are promising for treating arsenic contaminated waters. However, mechanisms of biogenic As-sulfide formation need to be better understood to decontaminate AMDs in acidic conditions. Here, we show that a high-As AMD effluent can be decontaminated by an indigenous SRB consortium. AMD water from the Carnoules mine (Gard, France) was incubated with the consortium under anoxic conditions and As, Zn and Fe concentrations, pH and microbial activity were monitored during 94 days. Precipitated solids were analyzed using electron microscopy (SEM/TEM-EDXS), and Extended X-Ray Absorption Fine Structure (EXAFS) spectroscopy at the As K-edge. Total removal of arsenic and zinc from solution (1.06 and 0.23 mmol/L, respectively) was observed in two of the triplicates. While Zn precipitated as ZnS nanoparticles, As precipitated as amorphous orpiment (am-(As2S3)-S-III) (33-73%), and realgar ((AsS)-S-II) (0-34%), the latter phase exhibiting a particular nanowire morphology. A minor fraction of As is also found as thiol-bound As-III (14-23%). We propose that the formation of the (AsS)-S-II nanowires results from (As2S3)-S-III reduction by biogenic H2S, enhancing the efficiency of As removal. The present description of As immobilization may help to set the basis for bioremediation strategies using SRB. (C) 2016 Elsevier B.V. All rights reserved.

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