Biodegradation of the aromatic fraction from petroleum diesel fuel by Oerskovia sp. followed by comprehensive GC x GC-TOF MS

JOURNAL OF HAZARDOUS MATERIALS

卷:363

Article

Ljesevic, Marija¹  Gojgic-Cvijovic, Gordana¹  Ieda, Teruyo²  Hashimoto, Shunji²  Nakano, Takeshi³  Bulatovic, Sandra⁴  Ilic, Mila¹  Beskoski, Vladimir⁴ 

[1] Univ Belgrade, Inst Chem Technol & Met, Njegoseva 12,POB 473, Belgrade 11000, Serbia

[2] Natl Inst Environm Studies, 16-2 Onogawa, Tsukuba, Ibaraki 3058506, Japan

[3] Osaka Univ, Res Ctr Environm Preservat, 2-4 Yamadaoka, Suita, Osaka, Japan

[4] Univ Belgrade, Fac Chem, Studentski Trg 16,POB 51, Belgrade 11158, Serbia

关键词: Petroleum diesel fuel;    PAHs;    Biodegradation;    Oerskovia sp.;    GC x GC-TOF MS;   

DOI  :  10.1016/j.jhazmat.2018.10.005

来源: Elsevier

【 摘 要 】

Polycyclic aromatic hydrocarbons (PAHs) from petroleum and fossil fuels are one of the most dominant pollutants in the environment. Since aromatic fraction from petroleum diesel fuel is mainly composed of PAHs, it is important to discover new microorganisms that can biodegrade these compounds. This article describes the biodegradation of the aromatic fraction separated from petroleum diesel fuel using the strain Oerskovia sp. CHP-ZH25 isolated from petroleum oil-contaminated soil. The biodegradation was monitored by gravimetry and GC x GC-TOF MS. An innovative method was applied to visualize degraded compounds in the data provided by a GC x GC-TOF MS. It was shown that Oerskovia sp. CHP-ZH25 degraded 77.4% based on gravimetric analysis within 30 days. Average rate of degradation was 14.4 mg/L/day, 10.5 mg/l/day and 4.0 mg/l/day from 0 to 10 day, 10-20 and 20-30 day, respectively. The order of PAH degradation based on decrease in peak volume after 30 days of incubation was as follows: dibenzothiophene derivatives > benzo[b]thiophene derivatives > naphthalene derivatives > acenaphthene derivatives > acenaphthylene/biphenyl derivatives > fluorene derivatives > phenanthrene/anthracene derivatives. Here we demonstrated that Oerskovia sp. CHP-ZH25 could potentially be a suitable candidate for use in bioremediation of environments polluted with different PAHs.

【 授权许可】

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