Toxicological mechanism of excessive copper supplementation: Effects on coloration, copper bioaccumulation and oxidation resistance in mud crab Scylla paramamosain

JOURNAL OF HAZARDOUS MATERIALS

卷:395

Article

Luo, Jiaxiang¹  Zhu, Tingting¹  Wang, Xuexi¹  Cheng, Xin¹  Yuan, Ye¹  Jin, Min¹  Betancor, Monica B.²  Tocher, Douglas R.²  Zhou, Qicun¹ 

[1] Ningbo Univ, Sch Marine Sci, Lab Fish & Shellfish Nutr, Ningbo 315211, Peoples R China

[2] Univ Stirling, Inst Aquaculture Fac Nat Sci, Stirling FK9 4LA, Scotland

关键词: Copper;    Coloration;    Oxidation resistance;    Mud crab Scylla paramamosain;   

DOI  :  10.1016/j.jhazmat.2020.122600

来源: Elsevier

【 摘 要 】

Copper is a widespread pollutant in marine environments, and marine animals can ingest large amounts of copper through the food chain. Here, an 8-week feeding trial was designed to investigate the effects of different dietary copper levels on coloration, copper bioaccumulation, stress response and oxidation resistance of Juvenile mud crab Scylla paramamosain. The results indicated that crabs fed the diet with 162 mg/kg copper exhibited a dark-blue carapace and hemolymph. The accumulation of copper in tissues was positively correlated with the level of copper in feed. High/excess dietary copper (162 mg/kg) up-regulated the expression of stress response related genes, and reduced the expression/activities of anti-oxidation genes/enzymes. The activity of phenoloxidase decreased significantly when dietary copper level was 86-162 mg/kg, and the expression of hemocyanin was up-regulated in crab fed the diets with 28-162 mg/kg copper. Overall, the results of the present study indicated that high dietary copper led to parachrea in carapace and hemoly mph of mud crab, and caused copper deposition abnormality in carapace and hepatopancreas. The data suggested that the toxic effects of dietary copper were concentration-dependent such that, excess dietary copper (162 mg/kg) had adverse impacts on oxidation resistance.

【 授权许可】

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