Study on the toxic effects of diphenol compounds on soil microbial activity by a combination of methods

JOURNAL OF HAZARDOUS MATERIALS

卷:167

Article

Chen, Huilun¹  Yao, Jun¹  Wang, Fei¹  Choi, Martin M. F.²  Bramanti, Emilia³  Zaray, Gyula⁴ 

[1] China Univ Geosci, Key Lab Biogeol & Environm Geol Lab, Chinese Minist Educ, Sch Environm Studies,Sinohungarian Joint Lab Envi, Wuhan 430074, Peoples R China

[2] Hong Kong Baptist Univ, Dept Chem, Hong Kong, Hong Kong, Peoples R China

[3] Inst Chem & Phys Proc, Lab Instrumental Analyt Chem, Area Ric, I-56124 Pisa, Italy

[4] Eotvos Lorand Univ, Dept Chem Technol & Environm Chem, H-1518 Budapest, Hungary

关键词: Plate counting;    Microcalorimetry;    Enzymatic activity;    Soil microbial activity;    Diphenols;   

DOI  :  10.1016/j.jhazmat.2009.01.066

来源: Elsevier

【 摘 要 】

Microcalorimetric technique based on heat-output measurement, direct microorganism counting and enzymatic activity determination, have been explored to evaluate the toxic effects of diphenol species (catechol, resorcinol, and hydroquinone) on soil microbial activity. The thermokinetic parameters including growth rate constant (k), inhibitory ratio, half inhibitory concentration and total thermal effect (Q(total)), were calculated and compared using the data obtained from the power-time curves of the microcalorimeter. It was found that addition of high concentrations of diphenol compounds to the soil samples resulted in low microorganism counts. The trend of the number of cultivable microorganisms with increasing concentration of diphenols was similar to specific growth rate k. It appeared that the higher the water soluble carbon (WSC) content, the higher the Q(total) value. The low dehydrogenase and beta-glucosidase found in the soils treated by catechol and hydroquinone was possibly due to their low WSC concentration and high inhibitory effects, respectively. The results reveal the toxicity of the three diphenols in a descending sequence: hydroquinone, resorcinol and catechol. The combination of the three methods is a more comprehensive toxicological investigation of a complex microbiological system. Microcalorimetry is for studying the metabolic growth of microorganisms, the plate counting method is for quantifying the real microbial growth, and the soil enzyme activity is for assessing the intracellular and extracellular activity of microbial biomass. Our proposed methods can provide toxicological information of diphenols to soil microbes from the metabolic, microbial and biochemical point of views which are consistent with and correlated to each other. Crown Copyright (C) 2009 Published by Elsevier B.V. All rights reserved.

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