Biotransformation of tetracycline by a novel bacterial strain Stenotrophomonas maltophilia DT1

JOURNAL OF HAZARDOUS MATERIALS

卷:318

Article

Leng, Yifei^1,4  Bao, Jianguo¹  Chang, Gaofeng²  Zheng, Han¹  Li, Xingxing¹  Du, Jiangkun¹  Snow, Daniel³  Li, Xu⁴ 

[1] China Univ Geosci, Sch Environm Studies, Wuhan 430074, Peoples R China

[2] Tianjin Environm Protect Tech Dev Ctr, Tianjin 300191, Peoples R China

[3] Univ Nebraska, Water Sci Lab, Lincoln, NE 68583 USA

[4] Univ Nebraska, Dept Civil Engn, Lincoln, NE 68588 USA

关键词: Tetracycline;    Biotransformation;    Hydrolysis;    Stenotrophomonas maltophilia;    Transformation products;   

DOI  :  10.1016/j.jhazmat.2016.06.053

来源: Elsevier

【 摘 要 】

Although several abiotic processes have been reported that can transform antibiotics, little is known about whether and how microbiological processes may degrade antibiotics in the environment. This work isolated one tetracycline degrading bacterial strain, Stenotrophomonas maltophilia strain DT1, and characterized the biotransformation of tetracycline by DT1 under various environmental conditions. The biotransformation rate was the highest when the initial pH was 9 and the reaction temperature was at 30 degrees C, and can be described using the Michaelis-Menten model under different initial tetracycline concentrations. When additional substrate was present, the substrate that caused increased biomass resulted in a decreased biotransformation rate of tetracycline. According to disk diffusion tests, the biotransformation products of tetracycline had lower antibiotic potency than the parent compound. Six possible biotransformation products were identified, and a potential biotransformation pathway was proposed that included sequential removal of N-methyl, carbonyl, and amine function groups. Results from this study can lead to better estimation of the fate and transport of antibiotics in the environment and has the potential to be utilized in designing engineering processes to remove tetracycline from water and soil. (C) 2016 Elsevier B.V. All rights reserved.

【 授权许可】

Free