Effects of gamma ionizing radiation exposure on Danio rerio embryo-larval stages - comparison with tritium exposure

JOURNAL OF HAZARDOUS MATERIALS

卷:408

Article

Gagnaire, Beatrice¹  Arcanjo, Caroline¹  Cavalie, Isabelle¹  Camilleri, Virginie¹  Simon, Olivier¹  Dubourg, Nicolas¹  Floriani, Magali¹  Adam-Guillermin, Christelle² 

[1] Inst Radioprotect & Surete Nucl IRSN, PSE ENV SRTE LECO, Cadarache, F-13115 St Paul Les Durance, France

[2] Inst Radioprotect & Surete Nucl IRSN, Cadarache, PSE SANTE SDOS LMDN, F-13115 Cadarache, France

关键词: Gamma irradiation;    Tritium;    Zebrafish, Danio rerio;    Embryo-larvae stages;    Molecular responses;   

DOI  :  10.1016/j.jhazmat.2020.124866

来源: Elsevier

【 摘 要 】

The objective was to investigate the effects of ionizing radiation induced in zebrafish early life stages by coupling responses obtained at the molecular (genotoxicity, ROS production, gene expression) and phenotypic (tissue alteration, embryo-larval development) levels. Here we present results obtained after exposure of 3 hpf larvae to 10 days of gamma irradiation at 3.3 x 10(1), 1.3 x 10(2) and 1.2 x 10(3) mu Gy/h, close to and higher than the benchmark for protection of ecosystems towards ionizing radiations of 10(1) mu Gy/h. Dose rates used in these studies were chosen to be in the 'derived consideration reference level' (DCRL) for gamma irradiation where deleterious effects can appear in freshwater fish. Also, these dose rates were similar to the ones already tested on tritium (beta ionizing radiation) in our previous work, in order to compare both types of ionizing radiation. Results showed that gamma irradiation did not induce any effect on survival and hatching. No effect was observed on DNA damages, but ROS production was increased. Muscle damages were observed for all tested dose rates, similarly to previous results obtained with tritium (beta ionizing radiation) at similar dose rates. Some molecular responses therefore appeared to be relevant for the study of gamma ionizing radiation effects in zebrafish.

【 授权许可】

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