Mitochondria-targeted acridine-based dual-channel fluorescence chemosensor for detection of Sn4+ and Cr2O72- ions in water and its application in discriminative detection of cancer cells

JOURNAL OF HAZARDOUS MATERIALS

卷:419

Article

Ravichandiran, Palanisamy^1,5  Prabakaran, D. S.^2,3  Maroli, Nikhil⁴  Kim, Ae Rhan⁵  Park, Byung-Hyun⁶  Han, Myung-Kwan⁷  Ramesh, Thiyagarajan⁸  Ponpandian, Samuel³  Yoo, Dong Jin^1,5 

[1] Jeonbuk Natl Univ, R&D Educ Ctr Whole Life Cycle R&D Fuel Cell Syst, Jeonju 54896, Jeollabuk Do, South Korea

[2] Chungbuk Natl Univ, Coll Med, Dept Radiat Oncol, Chungdae Ro 1, Cheongju 28644, Chungbuk, South Korea

[3] Ayya Nadar Janaki Ammal Coll Autonomous, Dept Biotechnol, Srivilliputhur Main Rd, Sivakasi 626124, Tamil Nadu, India

[4] Indian Inst Sci, Dept Phys, Ctr Condensed Matter Theory, Bangalore 560012, Karnataka, India

[5] Jeonbuk Natl Univ, Hydrogen & Fuel Cell Res Ctr, Convers Engn Grad Sch, Dept Energy Storage,Dept Life Sci, Jeonju 54896, Jeollabuk Do, South Korea

[6] Jeonbuk Natl Univ, Sch Med, Dept Biochem, 567 Baekje Daero, Jeonju Si 54896, Jeollabuk Do, South Korea

[7] Jeonbuk Natl Univ, Sch Med, Dept Microbiol, 567 Baekje Daero, Jeonju Si 54896, Jeollabuk Do, South Korea

[8] Prince Sattam Bin Abdulaziz Univ, Coll Med, Dept Basic Med Sci, Al Kharj 11942, Saudi Arabia

关键词: Tetravalent tin;    Dichromate;    Fluorescence sensor;    Cancer cells;    Intracellular imaging;   

DOI  :  10.1016/j.jhazmat.2021.126409

来源: Elsevier

【 摘 要 】

The goal of the present work was to fabricate a new low-cost, easy-to-prepare, dual-channel fluorescence chemosensor comprised of acridine-diphenylacetyl moieties (NDA) to enable remarkable Sn4+ detection in water and biological medium. The resulting NDA-Sn4+ complex was utilized for the distinguished identification of Cr2O72- ions from other anions and biomolecules. These investigations involve the absorption, fluorescence, and electrochemical methods for the detection of Sn4+ and Cr2O72- ions in pure water. The mechanism for NDA-mediated Sn4+ detection was experimentally determined by FT-IR, NMR titrations, mass (ESI) analyses, and DFT calculations. The obtained results indicate that the NDA chemosensor possessed excellent performance characteristics including good water solubility and compatibility, quick response time (less than 10 s), high sensitivity (Sn4+ = 0.268 mu M and Cr2O72- = 0.160 mu M), and selectivity against coexisting metals, anions, amino acids, and peptides. The chemosensor NDA induced negligible toxicity in live cells and was successfully utilized as a biomarker for the tracking of Sn4+ in human normal and cancer cells. More importantly, NDA demonstrates distinguished recognition of Sn4+ in human cancer cells rather than in normal live cells. Additionally, NDA was shown to act as a mitochondria-targeted probe in FaDu cells.

【 授权许可】

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