Visualizing and evaluating mitochondrial cysteine via near-infrared fluorescence imaging in cells, tissues and in vivo under hypoxia/ reperfusion stress

JOURNAL OF HAZARDOUS MATERIALS

卷:419

Article

Zhang, Xia^1,5  Zhang, Liangwei^1,3  Wang, Xiaoyan^1,4  Han, Xiaoyue^1,5  Huang, Yan^1,4  Li, Bowei^1,3  Chen, Lingxin^1,2,3 

[1] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Key Lab Coastal Environm Proc, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China

[2] Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China

[3] Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China

[4] Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China

[5] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

关键词: Fluorescent probe;    Cysteine;    Near-infrared imaging;    Hypoxia;    reperfusion;    Environmental stress;   

DOI  :  10.1016/j.jhazmat.2021.126476

来源: Elsevier

【 摘 要 】

Increasingly grim environmental pollutions are closely related with the occurrence and development of diseases. However, it's obscure how environmental stress disturbs the normal physiological process, and then how endogenous reactive species mend the cases. Hypoxia/reperfusion (H/R), a common and intractable injury in aquaculture and clinic, can induce oxidative stress and ultimately cause irreversible injury to organism. Cysteine (Cys) plays essential roles in maintaining transduction of numerous reactive species and redox homeostasis in subcellular structures, cells and organisms. A great deal of fluorescence research about Cys are focusing on development of selective probes but with poor exploration of the biofunction under environmental stress. Therefore, it is of great significance to examine the bio-effects of Cys against H/R stress. In the present work, we design a fluorescent probe BCy-AC for in situ detecting Cys, the unique Enol-Keto tautomerization of fluorophore BCy-Keto propels the reaction process which will improve the sensitivity and potential application performance of the probe. BCy-AC is conveniently applied to visualize Cys in HT-22 cells, zebrafish and mice tissues. Moreover, imaging results obtained from H/R models reveal that endogenous Cys changes with hypoxia and reperfusion time and Cys pretreatment effectively defend H/R injury in cells and in vivo.

【 授权许可】

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