Use of omic tools to assess methyl tert-butyl ether (MTBE) degradation in groundwater

JOURNAL OF HAZARDOUS MATERIALS

卷:378

Article

Kucharzyk, Katarzyna H.¹  Rectanus, Heather, V²  Bartling, Craig M.¹  Rosansky, Steve¹  Minard-Smith, Angela¹  Mullins, Larry A.¹  Neil, Kenda³ 

[1] Battelle Mem Inst, 505 King Ave, Columbus, OH 43201 USA

[2] Geosyntec, Columbia, MD USA

[3] Engn & Expeditionary Warfare Ctr EXWC, Naval Facil Engn Command NAVFAC, Port Huaneme, CA USA

关键词: MTBE;    Biodegradation;    Natural attenuation;    Metagenomics;    Proteomics;   

DOI  :  10.1016/j.jhazmat.2019.05.011

来源: Elsevier

【 摘 要 】

This study employed innovative technologies to evaluate multiple lines of evidence for natural attenuation (NA) of methyl tertiary-butyl ether (MTBE) in groundwater at the 22 Area of Marine Corps Base (MCB) Camp Pendleton after decommissioning of a biobarrier system. For comparison, data from the 13 Area Gas Station where active treatment of MTBE is occurring was used to evaluate the effectiveness of omic techniques in assessing biodegradation. Overall, the 22 Area Gas Station appeared to be anoxic. MTBE was detected in large portion of the plume. In comparison, concentrations of MTBE at the 13 Area Gas Station were much higher (42,000 mu g/L to 2800 mu g/L); however, none of the oxygenates were detected. Metagenomic analysis of the indigenous groundwater microbial community revealed the presence of bacterial strains known to aerobically and anaerobically degrade MTBE at both sites. While proteomic analysis at the 22 Area Gas Station showed the presence of proteins of MTBE degrading microorganisms, the MTBE degradative proteins were only found at the 13 Area Gas Station. Taken together, these results provide evidence for previous NA of MTBE in the groundwater at 22 Area Gas Station and demonstrate the effectiveness of innovative-omic technologies to assist monitored NA assessments.

【 授权许可】

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