Optimization of process parameters influencing the sustainable construction of iron oxide nanoparticles by a novel tropical wetlands Streptomyces spp.

JOURNAL OF CLEANER PRODUCTION

卷:232

Article

Jacob, Patricia Jayshree^1,4  Masarudin, Mas Jaffri^1,2,3  Hussein, Mohd Zobir³  Rahim, Raha Abdul¹ 

[1] Univ Putra Malaysia, Fac Biotechnol & Biomol Sci, Dept Cell & Mol Biol, Upm Serdang 43400, Selangor, Malaysia

[2] Univ Putra Malaysia, Inst Biosci, Upm Serdang 43400, Selangor, Malaysia

[3] Univ Putra Malaysia, Inst Adv Technol, Upm Serdang 43400, Selangor, Malaysia

[4] Persiaran Univ, Nilai Univ, Dept Biosci, Negeri Sembilan 71800, Malaysia

关键词: Melanin induced;    Bionanoparticles;    Optimization;    Size;    Polydispersity;    Temperature;    Biomass;    Precursor concentration;    Reaction kinetics;    Iron oxide nanoparticles;    External parameters;    Microbial mediated;    RSM;    OVAT analysis;   

DOI  :  10.1016/j.jclepro.2019.05.359

来源: Elsevier

【 摘 要 】

A Streptomyces strain isolated from the soil sediments of tropical freshwater wetlands in Malaysia demonstrated promising attributes to be developed into a versatile microbial nanofactory for the sustainable synthesis of ferric oxide nanoparticles (IONP). Process parameters such as temperature, ferric salt precursor concentration, cell free extract (CFE) concentration and biomass harvesting times are serious players in the extracellular generation of metallic nanoparticles. A statistical approach using One Variable -At-A-Time (OVAT) Analysis followed by Response Surface Methodology (RSM) was employed towards the optimization of the microbial bioprocess and modulation of nanoparticle size dimensions. OVAT revealed that IONP production increased with increasing temperature, precursor concentration, harvesting time and CFE concentration with highest yield at 65 degrees C, 2 mM precursor concentration, 68 h harvesting time and 100% CFE concentration. A detailed statistical analysis using RSM (RSM) showed significantly strong negative interactive effects between temperature and CFE concentration (p = 0.0037), temperature and precursor concentration (p = 0.0093) and mild interactive effects between CFE and precursor concentration (p = 0.0301). Taking into account the interactive influence of these variables, numerical analysis using RSM proposed that for optimal generation of microbial mediated IONP, a CFE concentration of 55.58%, temperature of 55.75 degrees C and precursor concentration of 2.46 mM FeCl3 center dot 6H(2)O would be required. (C) 2019 Elsevier Ltd. All rights reserved.

【 授权许可】

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