Energy-efficient and environmentally friendly production of starch-rich duckweed biomass using nitrogen-limited cultivation

JOURNAL OF CLEANER PRODUCTION

卷:251

Article

Guo, Ling^1,2  Jin, Yanling^1,2  Xiao, Yao³  Tan, Li^1,2  Tian, Xueping^1,2,4  Ding, Yanqiang^1,2,4  He, Kaize^1,2  Du, Anping^1,2  Li, Jinmeng^1,2,4  Yi, Zhuolin^1,2  Wang, Songhu⁵  Fang, Yang^1,2  Zhao, Hai^1,2 

[1] Chinese Acad Sci, Chengdu Inst Biol, Key Lab Environm & Appl Microbiol, Chengdu 610041, Peoples R China

[2] Environm Microbiol Key Lab Sichuan Prov, Chengdu 610041, Peoples R China

[3] Sichuan Univ Sci & Engn, Analyt & Testing Ctr, Zigong 643000, Peoples R China

[4] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

[5] Chinese Acad Sci, CAS Ctr Excellence Mol Plant Sci, Chengdu Inst Biol, Chengdu 610041, Peoples R China

关键词: Duckweed;    Nitrogen limitation;    Nitrogen use efficiency;    Glutamine synthase;    Starch production;   

DOI  :  10.1016/j.jclepro.2019.119726

来源: Elsevier

【 摘 要 】

Here, we combined the aquatic cultivation of the energy crop duckweed and nitrogen limitation to quickly produce starch-rich biomass. Under N limitation, duckweed can quickly accumulate starch at up to 52.37 +/- 0.57% (dry weight). In the process, nitrogen use efficiency (NUE) reached 61.3 +/- 1.8 kg biomass kg(-1) N, exceeding that of most cereals. In this paper, we elucidated for the first time the mechanism of the high NUE of duckweed under N limitation. First, duckweed possesses higher N transport ability than most cereals and trees and can efficiently accumulate abundant N in vivo under N-sufficient condition. In addition, under N limitation, the stable expression and activity of key enzymes related to both carbon fixation and N assimilation ensured normal biomass accumulation. Furthermore, the efficient ubiquitin-proteasome system (UPS) and autophagy activated the endogenous N remobilization and recycling. Combined with the sharp increase in the expression and activity of duckweed glutamine synthase (GS), duckweed can efficiently redistribute and reuse the endogenous N to support its growth and starch accumulation. Especially, sewage was used to duckweed pre-cultivation in pilot-scale experiment, the approach can simultaneously achieve starch production and wastewater treatment. In a word, this paper provides an environmentally friendly, cost-effective and promising technology for starch production. (C) 2019 The Authors. Published by Elsevier Ltd.

【 授权许可】

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