Hydrogen production during direct cellulose fermentation by mixed bacterial culture: The relationship between the key process parameters using response surface methodology

JOURNAL OF CLEANER PRODUCTION

卷:314

Article

Zagrodnik, Roman¹  Duber, Anna²  Seifert, Krystyna¹ 

[1] Adam Mickiewicz Univ, Fac Chem, Uniwersytetu Poznanskiego 8, PL-61614 Poznan, Poland

[2] Poznan Univ Tech, Fac Civil & Environm Engn, Inst Environm Engn, Berdychowo 4, PL-60965 Poznan, Poland

关键词: Hydrogen;    Cellulose;    Box-Behnken design;    Anaerobic sludge;    Enrichment;    Dark fermentation;   

DOI  :  10.1016/j.jclepro.2021.127971

来源: Elsevier

【 摘 要 】

Dark fermentation is a promising method to produce hydrogen from lignocellulosic biomass. This study assessed the influence of temperature, phosphate buffer concentration and substrate concentration on direct hydrogen production form cellulose using response surface methodology. Mixed bacterial culture was successfully enriched on cellulose and used as an inoculum for hydrogen production. The model indicated that the highest cumulative hydrogen production (CHP) of 2.14 L H-2/L-medium could be obtained at 13.5 g(cellulose)/L, 79.5 mM buffer and 32.6 degrees C. However, hydrogen yield is then only 0.58 mol H-2/mol(hexose) due to low substrate conversion efficiency (SCE). Simultaneous optimization of CHP and SCE with desirability function approach resulted in the H-2 yield of 2.71 +/- 0.1 mol H-2/mol(hexose) and 93.8 +/- 1.8% SCE at 3.35 g(cellulose)/L, 69 mM buffer and 32.9 degrees C. Phosphate concentration above 80 mM decreased H-2 production, but had positive effect on cellulose consumption. The bacterial community analysis showed that Ruminiclostridium papyrosolvens was responsible for cellulose hydrolysis. Lachnoclostridium sp. was positively correlated with ethanol production at high phosphate buffer concentration, while Caproiciproducens sp. with caproate production at low buffer concentration. The obtained results opens the possibility of simultaneous hydrogen and caproate production from cellulosic substrates.

【 授权许可】

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