MUTATIONAL ANALYSIS OF THE CYTOPLASMIC LINKER REGION OF TAZ1-1, A TAR-ENVZ CHIMERIC RECEPTOR IN ESCHERICHIA-COLI

JOURNAL OF MOLECULAR BIOLOGY

卷:244

Note

JIN, T ; INOUYE, M

关键词: TRANSMEMBRANE SIGNALING;    HISTIDINE KINASE;    PHOSPHATASE;    CHEMOSENSOR;    RECEPTOR;   

DOI  :  10.1006/jmbi.1994.1746

来源: Elsevier

【 摘 要 】

Taz1-1 is Tar-EnvZ chimeric receptor that is able to induce ompC-lacZ expression in response to aspartate. Previous studies indicated that aspartate binding to the receptor domain of the Taz1-1 receptor modulated the ratio of kinase and phosphatase activities of the cytoplasmic signaling domain. The 80-residue segment of chemoreceptors that is located between the second transmembrane domain and the signaling domain was defined as the linker region. The Taz1-1 chimeric receptor contains 43 amino acid residues of the Tar linker region. In order to understand further the function of the linker region in transmembrane signaling, site-directed random mutagenesis was carried out on the conserved Ala231 in the linker region. Substitution mutations with Val, Glu, Gly, Thr, Lys and His gave the locked ''off-mode'' form (low ompC-lacZ expression), and substitution mutations with Ile and Leu resulted in the locked ''on-mode'' form (constitutive ompC-lacZ expression). All the mutant Taz1-1 receptors still retained both OmpR kinase and phospho-OmpR phosphatase activities. Interestingly Taz1N6, a kinase defective mutant, was able to complement with TazlH1, a phosphatase defective mutant, carrying an off-mode mutant at position 231 to restore asp-inducible ompC-lacZ expression, but not with Taz1H1 carrying an on-mode mutation. These results suggest that the residue at position 231 in Taz1-1 plays a key role in signal transduction.

【 授权许可】

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