The Brace for a Growing Scaffold: Mss116 Protein Promotes RNA Folding by Stabilizing an Early Assembly Intermediate

JOURNAL OF MOLECULAR BIOLOGY

卷:422

Article

Pyle, Anna Marie¹ 

[1] Yale Univ, Howard Hughes Med Inst, New Haven, CT 06520 USA

关键词: ribozyme;    RNA folding;    DEAD-box protein;    NAIM;   

DOI  :  10.1016/j.jmb.2012.05.037

来源: Elsevier

【 摘 要 】

The ai5 gamma group II intron requires a protein cofactor to facilitate native folding in the cell. Yeast protein Mss116 greatly accelerates intron folding under near-physiological conditions both in vivo and in vitro. Although the effect of Mss116 on the kinetics of ai5 gamma ribozyme folding and catalysis has been extensively studied, the precise structural role and interaction sites of Mss116 have been elusive. Using Nucleotide Analog Interference Mapping to study the folding of splicing precursor constructs, we have identified specific intron functional groups that participate in Mss116-facilitated folding and we have determined their role in the folding mechanism. The data indicate that Mss116 stabilizes an early, obligate folding intermediate within intron domain 1, thereby laying the foundation for productive folding to the native state. In addition, the data reveal an important role for the IBS2 exon sequence and for the terminus of domain 6, during the folding of self-splicing group JIB intron constructs. (c) 2012 Published by Elsevier Ltd.

【 授权许可】

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