The HMGB1 C-Terminal Tail Regulates DNA Bending

JOURNAL OF MOLECULAR BIOLOGY

卷:428

Article

Blair, Rebecca H.^1,2  Horn, Abigail E.¹  Pazhani, Yogitha¹  Grado, Lizbeth¹  Goodrich, James A.¹  Kugel, Jennifer F.¹ 

[1] Univ Colorado, Dept Chem & Biochem, 596 UCB, Boulder, CO 80309 USA

[2] InDevR Inc, 2100 Cent Ave,Suite 106, Boulder, CO 80301 USA

关键词: FRET;    single-molecule;    TIRF microscopy;    DNA bending;    HMGB1;   

DOI  :  10.1016/j.jmb.2016.08.018

来源: Elsevier

【 摘 要 】

High mobility group box protein 1 (HMGB1) is an architectural protein that facilitates the formation of protein DNA assemblies involved in transcription, recombination, DNA repair, and chromatin remodeling. Important to its function is the ability of HMGB1 to bend DNA non-sequence specifically. HMGB1 contains two HMG boxes that bind and bend DNA (the A box and the B box) and a C-terminal acidic tail. We investigated how these domains contribute to DNA bending by HMGB1 using single-molecule fluorescence resonance energy transfer (FRET), which enabled us to resolve heterogeneous populations of bent and unbent DNA. We found that full-length (FL) HMGB1 bent DNA more than the individual A and B boxes. Removing the C-terminal tail resulted in a protein that bent DNA to a greater extent than the FL protein. These data suggest that the A and B boxes simultaneously bind DNA in the absence of the C-terminal tail, but the tail modulates DNA binding and bending by one of the HMG boxes in the FL protein. Indeed, a construct composed of the B box and the C-terminal tail only bent DNA at higher protein concentrations. Moreover, in the context of the FL protein, mutating the A box such that it could not bend DNA resulted in a protein that bent DNA similar to a single HMG box and only at higher protein concentrations. We propose a model in which the HMGB1 C-terminal tail serves as an intramolecular damper that modulates the interaction of the B box with DNA. (C) 2016 Elsevier Ltd. All rights reserved.

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