Globins Synthesize the Second Messenger Bis-(3′-5′)-Cyclic Diguanosine Monophosphate in Bacteria

JOURNAL OF MOLECULAR BIOLOGY

卷:388

Article

Wan, Xuehua¹  Tuckerman, Jason R.²  Saito, Jennifer A.¹  Freitas, Tracey Allen K.¹  Newhouse, James S.³  Denery, Judith R.⁴  Galperin, Michael Y.⁵  Gonzalez, Gonzalo²  Gilles-Gonzalez, Marie-Alda²  Alam, Maqsudul^1,3,6 

[1] Univ Hawaii, Dept Microbiol, Honolulu, HI 96822 USA

[2] Univ Texas SW Med Ctr Dallas, Dept Biochem, Dallas, TX 75390 USA

[3] Maui High Performance Comp Ctr, Maui, HI 96753 USA

[4] Univ Hawaii, Dept Mol Biosci & Bioengn, Honolulu, HI 96822 USA

[5] NIH, Natl Ctr Biotechnol Informat, Natl Lib Med, Bethesda, MD 20894 USA

[6] Univ Hawaii, Adv Studies Genom Prote & Bioinformat, Honolulu, HI 96822 USA

关键词: globin;    oxygen sensor;    c-di-GMP;    diguanylate cyclase;    biofilm;   

DOI  :  10.1016/j.jmb.2009.03.015

来源: Elsevier

【 摘 要 】

Globin-coupled sensors are heme-bolding signal transducers in Bacteria and Archaea in which an N-terminal globin controls the activity of a variable C-terminal domain. Here, we report that BpeGReg, a globin-coupled diguanylate cyclase from the whooping cough pathogen Bordetella pertussis, synthesizes the second messenger bis-(3'-5')-cyclic diguanosine monophosphate (c-di-GMP) upon oxygen binding. Expression of BpeGReg m Salmonella typhimurium enhances biofilm formation, while knockout of the BpeGReg gene of B. pertussis results in decreased biofilm formation. These results represent the first identification a signal ligand for any diguanylate cyclase and provide definitive experimental evidence that a globin-coupled sensor regulates c-di-GMP synthesis and biofilm formation. We propose that the synthesis of c-di-GMP by globin sensors is a widespread phenomenon in bacteria. (C) 2009 Elsevier Ltd. All rights reserved.

【 授权许可】

Free