A New Yeast Peroxin, Pex36, a Functional Homolog of Mammalian PEX16, Functions in the ER-to-Peroxisome Traffic of Peroxisomal Membrane Proteins

JOURNAL OF MOLECULAR BIOLOGY

卷:429

Article

Farre, Jean-Claude¹  Carolino, Krypton¹  Stasyk, Oleh V.²  Stasyk, Olena G.^2,3  Hodzic, Zlatan¹  Agrawal, Gaurav¹  Till, Andreas⁴  Proietto, Marco¹  Cregg, James⁵  Sibirny, Andriy A.^2,6  Subramani, Suresh¹ 

[1] Univ Calif San Diego, Div Biol Sci, Mol Biol Sect, 9500 Gilman Dr,Bonner Hall 3326, La Jolla, CA 92093 USA

[2] Natl Acad Sci Ukraine, Inst Cell Biol, Drahomanov St 14-16, UA-79005 Lvov, Ukraine

[3] Ivan Franko Natl Univ Lviv, Fac Biol, Dept Biochem, Hrushevsky St 4, UA-79005 Lvov, Ukraine

[4] Univ Bonn, Inst Reconstruct Neurobiol, Fac Med, Sigmund Freud Str 25, D-53105 Bonn, Germany

[5] Keck Grad Inst Appl Life Sci, 535 Watson Dr, Claremont, CA 91711 USA

[6] Univ Rzeszow, Zelwerowicza 4, PL-35601 Rzeszow, Poland

关键词: peroxin;    PMP trafficking;    pre-peroxisomal vesicle formation;    peroxisome biogenesis;    ER;   

DOI  :  10.1016/j.jmb.2017.10.009

来源: Elsevier

【 摘 要 】

Peroxisomal membrane proteins (PMPs) traffic to peroxisomes by two mechanisms: direct insertion from the cytosol into the peroxisomal membrane and indirect trafficking to peroxisomes via the endoplasmic reticulum (ER). In mammals and yeast, several PMPs traffic via the ER in a Pex3- and Pex19-dependent manner. In Komagataella phaffii (formerly called Pichia pastoris) specifically, the indirect traffic of Pex2, but not of Pex11 or Pex17, depends on Pex3, but all PMPs tested for indirect trafficking require Pex19. In mammals, the indirect traffic of PMPs also requires PEX16, a protein that is absent in most yeast species. In this study, we isolated PEX36, a new gene in K. phaffii, which encodes a PMP. Pex36 is required for cell growth in conditions that require peroxisomes for the metabolism of certain carbon sources. This growth defect in cells lacking Pex36 can be rescued by the expression of human PEX16, Saccharomyces cerevisiae Pex34, or by overexpression of the endogenous K. phaffii Pex25. Pex36 is not an essential protein for peroxisome proliferation, but in the absence of the functionally redundant protein, Pex25, it becomes essential and less than 20% of these cells show import-incompetent, peroxisome-like structures (peroxisome remnants). In the absence of both proteins, peroxisome biogenesis and the intra-ER sorting of Pex2 and Pex11C are seriously impaired, likely by affecting Pex3 and Pex19 function. (C) 2017 Elsevier Ltd. All rights reserved.

【 授权许可】

Free