Single-Molecule Studies of Transcription: From One RNA Polymerase at a Time to the Gene Expression Profile of a Cell

JOURNAL OF MOLECULAR BIOLOGY

卷:412

Review

Wang, Feng¹  Greene, Eric C.^1,2 

[1] Columbia Univ, Dept Biochem & Mol Biophys, New York, NY 10032 USA

[2] Columbia Univ, Howard Hughes Med Inst, New York, NY 10032 USA

关键词: single molecule;    transcription;    RNA polymerase;   

DOI  :  10.1016/j.jmb.2011.01.024

来源: Elsevier

【 摘 要 】

Single-molecule techniques have emerged as powerful tools for deciphering mechanistic details of transcription and have yielded discoveries that would otherwise have been impossible to make through the use of more traditional biochemical and/or biophysical techniques. Here, we provide a brief overview of single-molecule techniques most commonly used for studying RNA polymerase and transcription. We then present specific examples of single-molecule studies that have contributed to our understanding of key mechanistic details for each different stage of the transcription cycle. Finally, we discuss emerging single-molecule approaches and future directions, including efforts to study transcription at the single-molecule level in living cells. (C) 2011 Elsevier Ltd. All rights reserved.

【 授权许可】

Free