Site-Specific Structural Variations Accompanying Tubular Assembly of the HIV-1 Capsid Protein

JOURNAL OF MOLECULAR BIOLOGY

卷:426

Article

Bayro, Marvin J.¹  Chen, Bo²  Yau, Wai-Ming¹  Tycko, Robert¹ 

[1] NIDDK, Chem Phys Lab, NIH, Bethesda, MD 20892 USA

[2] Univ Cent Florida, Dept Phys, Orlando, FL 32816 USA

关键词: human immunodeficiency virus;    AIDS;    solid-state NMR;    electron microscopy;    automated resonance assignment;   

DOI  :  10.1016/j.jmb.2013.12.021

来源: Elsevier

【 摘 要 】

The 231-residue capsid (CA) protein of human immunodeficiency virus type 1 (HIV-1) spontaneously self-assembles into tubes with a hexagonal lattice that is believed to mimic the surface lattice of conical capsid cores within intact virions. We report the results of solid-state nuclear magnetic resonance (NMR) measurements on HIV-1 CA tubes that provide new information regarding changes in molecular structure that accompany CA self-assembly, local dynamics within CA tubes, and possible mechanisms for the generation of lattice curvature. This information is contained in site-specific assignments of signals in two- and three-dimensional solid-state NMR spectra, conformation-dependent N-15 and C-13 NMR chemical shifts, detection of highly dynamic residues under solution NMR conditions, measurements of local variations in transverse spin relaxation rates of amide H-1 nuclei, and quantitative measurements of site-specific N-15-N-15 dipole dipole couplings. Our data show that most of the CA sequence is conformationally ordered and relatively rigid in tubular assemblies and that structures of the N-terminal domain (NTD) and the C-terminal domain (CTD) observed in solution are largely retained. However, specific segments, including the N-terminal 13-hairpin, the cyclophilin A binding loop, the inter-domain linker, segments involved in intermolecular NTD CTD interactions, and the C-terminal tail, have substantial static or dynamical disorder in tubular assemblies. Other segments, including the 310-helical segment in CTD, undergo clear conformational changes. Structural variations associated with curvature of the CA lattice appear to be localized in the inter-domain linker and intermolecular NTD CTD interface, while structural variations within NTD hexamers, around local 3-fold symmetry axes, and in CTD CTD dimerization interfaces are less significant. Published by Elsevier Ltd.

【 授权许可】

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