Exocyst Subcomplex Functions in Autophagosome Biogenesis by Regulating Atg9 Trafficking

JOURNAL OF MOLECULAR BIOLOGY

卷:431

Article

Singh, Sunaina¹  Kumari, Ruchika¹  Chinchwadkar, Sarika¹  Aher, Amol¹  Matheshwaran, Saravanan²  Manjithaya, Ravi¹ 

[1] Jawaharlal Nehru Ctr Adv Sci Res, Mol Biol & Genet Unit, Bangalore 560064, Karnataka, India

[2] Indian Inst Technol, Dept Biol Sci & Bioengn, Kanpur 208016, Uttar Pradesh, India

关键词: Exocyst;    Autophagosome biogenesis;    Atg9 trafficking;    Autophagy;    Tethers;   

DOI  :  10.1016/j.jmb.2019.04.048

来源: Elsevier

【 摘 要 】

During autophagy, double-membrane vesicles called autophagosomes capture and degrade the intracellular cargo. The de novo formation of autophagosomes requires several vesicle transport and membrane fusion events which are not completely understood. We studied the involvement of exocyst, an octameric tethering complex, which has a primary function in tethering post-Golgi secretory vesicles to plasma membrane, in autophagy. Our findings indicate that not all subunits of exocyst are involved in selective and general autophagy. We show that in the absence of autophagy specific subunits, autophagy arrest is accompanied by accumulation of incomplete autophagosome-like structures. In these mutants, impaired Atg9 trafficking leads to decreased delivery of membrane to the site of autophagosome biogenesis thereby impeding the elongation and completion of the autophagosomes. The subunits of exocyst, which are dispensable for autophagic function, do not associate with the autophagy specific subcomplex of exocyst. (C) 2019 The Authors. Published by Elsevier Ltd.

【 授权许可】

Free