miREV: An Online Database and Tool to Uncover Potential Reference RNAs and Biomarkers in Small-RNA Sequencing Data Sets from Extracellular Vesicles Enriched Samples

JOURNAL OF MOLECULAR BIOLOGY

卷:433

Article

Hildebrandt, Alex¹  Kirchner, Benedikt¹  Nolte-'t Hoen, Esther N. M.²  Pfaffl, Michael W.¹ 

[1] Tech Univ Munich, Anim Physiol & Immunol, Freising Weihenstephan, Germany

[2] Univ Utrecht, Dept Biochem & Cell Biol, Utrecht, Netherlands

关键词: biofluid;    normalization methods;    stability algorithms;    human blood plasma & serum;    RT-qPCR;    Extracellular vesicles;    Next-generation Sequencing;   

DOI  :  10.1016/j.jmb.2021.167070

来源: Elsevier

【 摘 要 】

Extracellular vesicles (EVs) are nano-sized, membrane-enclosed vesicles released by cells for intercellular communication. EVs are involved in pathological processes and miRNAs in EVs have gained interest as easily accessible biomolecules in liquid biopsies for diagnostic purposes. To validate potential miRNA biomarker, transcriptome analyses must be carried out to detect suitable reference miRNAs. miREV is a database with over 400 miRNA sequencing data sets and helps the researcher to find suitable reference miRNAs for their individual experimental setup. The researcher can put together a specific sample set in miREV, which is similar to his own experimental concept in order to find the most suitable references. This allows to run validation experiments without having to carry out a complex and costly transcriptome analysis priorly. Additional read count tables of each generated sample set are downloadable for further analysis. miREV is freely available at https://www.physio.wzw.tum.de/mirev/. (C) 2021 Published by Elsevier Ltd.

【 授权许可】

Free