The Pierced Lasso Topology Leptin has a Bolt on Dynamic Domain Composed by the Disordered Loops I and III

JOURNAL OF MOLECULAR BIOLOGY

卷:432

Article

Danielsson, Jens¹  Noel, Jeffrey Kenneth²  Simien, Jennifer Michelle³  Duggan, Brendan Michael⁴  Oliveberg, Mikael¹  Onuchic, Jose Nelson^5,6  Jennings, Patricia Ann⁷  Haglund, Ellinor³ 

[1] Stockholm Univ, Dept Biochem & Biophys, Stockholm, Sweden

[2] Max Delbruck Ctr Mol Med, Kristallog, Berlin, Germany

[3] Univ Hawaii, Dept Chem, Honolulu, HI 96822 USA

[4] Univ Calif San Diego, Skaggs Sch Pharm & Pharmaceut Sci, La Jolla, CA 92093 USA

[5] Rice Univ, Ctr Theoret Biol Phys, Houston, TX USA

[6] Rice Univ, Dept Chem, Dept Phys & Astron, Houston, TX USA

[7] Univ Calif San Diego, Dept Chem & Biochem, La Jolla, CA 92093 USA

关键词: conformational dynamics;    NMR;    protein folding;    obesity;    leptin;   

DOI  :  10.1016/j.jmb.2020.01.035

来源: Elsevier

【 摘 要 】

Leptin is an important signaling hormone, mostly known for its role in energy expenditure and satiety. Furthermore, leptin plays a major role in other proteinopathies, such as cancer, marked hyperphagia, impaired immune function, and inflammation. In spite of its biological relevance in human health, there are no NMR resonance assignments of the human protein available, obscuring high-resolution characterization of the soluble protein and/or its conformational dynamics, suggested as being important for receptor interaction and biological activity. Here, we report the nearly complete backbone resonance assignments of human leptin. Chemical shift-based secondary structure prediction confirms that in solution leptin forms a four-helix bundle including a pierced lasso topology. The conformational dynamics, determined on several timescales, show that leptin is monomeric, has a rigid four-helix scaffold, and a dynamic domain, including a transiently formed helix. The dynamic domain is anchored to the helical scaffold by a secondary hydrophobic core, pinning down the long loops of leptin to the protein body, inducing motional restriction without a well-defined secondary or tertiary hydrogen bond stabilized structure. This dynamic region is well suited for and may be involved in functional allosteric dynamics upon receptor binding. (C) 2020 Elsevier Ltd. All rights reserved.

【 授权许可】

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