Predicting knot or catenane type of site-specific recombination products

JOURNAL OF MOLECULAR BIOLOGY

卷:374

Article

Buck, Dorothy^1,2  Flapan, Erica³ 

[1] Univ London Imperial Coll Sci Technol & Med, Dept Math, London SW7 2AZ, England

[2] Univ London Imperial Coll Sci Technol & Med, Ctr Bioinformat, London SW7 2AZ, England

[3] Pomona Coll, Dept Math, Claremont, CA 91711 USA

关键词: site-specific recombination;    DNA knots;    serine recombinases;    tyrosine recombinases;    DNA topology;   

DOI  :  10.1016/j.jmb.2007.10.016

来源: Elsevier

【 摘 要 】

Site-specific recombination on supercoiled circular DNA yields a variety of knotted or catenated products. Here, we present a topological model of this process and characterize all possible products of the most common substrates: unknots, unlinks, and torus knots and catenanes. This model tightly prescribes the knot or catenane type of previously uncharacterized data. We also discuss how the model helps to distinguish products of distributive recombination and, in some cases, determine the order of processive recombination products. (c) 2007 Elsevier Ltd. All rights reserved.

【 授权许可】

Free