The entropy cost of protein association

JOURNAL OF MOLECULAR BIOLOGY

卷:273

Article

Tamura, A ; Privalov, PL

关键词: protein association;    translational entropy;    cratic entropy;    crosslinking;    subtilisin inhibitor;   

DOI  :  10.1006/jmbi.1997.1368

来源: Elsevier

【 摘 要 】

The temperature induced unfolding/dissociation of the dimeric subtilisin inhibitor from Streptomyces and its mutant D83C having an S-S crosslink between the subunits has been studied calorimetrically. Comparison of the entropies measured at different concentrations of dimer showed that the entropy cost of crosslinking is small. Its value at the standard concentration of 1 M is of the order of -(5 +/- 4) cal/K-mol, i.e. it is more than one order of magnitude smaller than the values of translational entropies calculated on the base of statistical thermodynamics, using in particular the Sackur-Tetrode equation, and is close to the cratic entropy value suggested by classical mixing theory. (C) 1997 Academic Press Limited.

【 授权许可】

Free