Migratory Langerhans cells in mouse lymph nodes in steady state and inflammation

JOURNAL OF INVESTIGATIVE DERMATOLOGY

卷:125

Article

Stoitzner, P ; Tripp, CH ; Douillard, P ; Saeland, S ; Romani, N

关键词: cell surface molecules;    cytokines;    dendritic cells;    inflammation;    skin;    steady state;   

DOI  :  10.1111/j.0022-202X.2005.23757.x

来源: Elsevier

【 摘 要 】

Dendritic cells cells induce immunity or-in the steady state-maintain peripheral tolerance. Little is known In that regard about Langerhans cells. Therefore, we investigated migrating Langerhans cells in the steady-state versus inflammation. Increased numbers of Langerhans cells, as determined by immunostaining for Langerin/CD207, appeared in the lymph nodes in response to a contact allergen. Whereas a large proportion of Langerhans cells expressed CD86 in the steady state, CD40, and CD80 were found on a smaller percentage. During inflammation, more CD40(+), CD80(+), CD274/B7-H1/PD-L1(+), and CD273JB7-DC/PD-L2(+) Langerhans cells were found in the lymph nodes, and they expressed higher levels of these molecules. CD275/inducible T cell co-stimulator (ICOS) ligand was not detected. Langerhans cells in the nodes of contact allergen-treated mice produced more IL-12p40/70. This correlated with more interferon-gamma being produced by activated lymph node T cells. Epicutaneous immunization with ovalbumin under inflammatory conditions led to a more vigorous proliferation of antigen-specific CD4 T cells in vitro and in vivo as compared with immunization in the steady state. The latter modality, however did not Induce strong CD4 T cell tolerance in this model. Thus, the overall phenotype of Langerhans cells is not an indicator for their immunogenic or tolerogenic potential.

【 授权许可】

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