Interleukin-10-treated dendritic cells modulate immune responses of naive and sensitized T cells in vivo

JOURNAL OF INVESTIGATIVE DERMATOLOGY

卷:119

Article

Müller, G ; Müller, A ; Tüting, T ; Stembrink, K ; Saloga, J ; Szalma, C ; Knop, J ; Enk, AH

关键词: dendritic cells;    interleukin-10;    ovalbumin;   

DOI  :  10.1046/j.1523-1747.2002.00496.x

来源: Elsevier

【 摘 要 】

Interleukin-10 is a pleiotropic cytokine known to have inhibitory effects on the accessory functions of dendritic cells. In vitro, interleukin-10 converts immature dendritic cells into tolerizing antigen-presenting cells. To assess whether interleukin-10-treated dendritic cells exert tolerizing effects in vivo, CD4(+) T cells from DO11.10 ovalbumin-T cell receptor transgenic mice were transferred to syngeneic BALB/c recipients. Recipient animals were treated with ovalbumin-pulsed/unpulsed, interleukin-10-treated/untreated CD11c(+) dendritic cells thereafter and ovalbumin-specific proliferation of lymph node cells was assessed by restimulation with the peptide in vitro. In prophylactic experiments, recipients received naive CD4(+) DO11.10 T cells and were immunized with ovalbumin(323-339) peptide in incomplete Freund's adjuvant after treatment with various subtypes of dendritic cells. Strong ovalbumin-specific proliferation was observed in animals immunized with control ovalbumin-dendritic cells. Minimal proliferation was found in mice treated with ovalbumin-pulsed, interleukin-10-treated dendritic cells. In therapeutic experiments, preactivated CD4(+) DO11.10 T cells were transferred, and recipients were treated with dendritic cells as described. Ovalbumin-specific proliferation was strong in recipients treated with ovalbumin-dendritic cells. CD4(+) T cell proliferation from ovalbumin-interleukin-10-dendritic cell treated animals was below background. When delayed type hypersensitivity reactions in the footpads of prophylactically or therapeutically vaccinated animals were tested, mice treated with ovalbumin-interleukin-10-dendritic cells showed no footpad swelling compared with controls. Rechallenge with the antigen in vitro and in vivo did not alter the inhibitory effect of interleukin-10-treated dendritic cells. Thus, interleukin-10-treated dendritic cells inhibit ovalbumin-specific immune responses in naive and sensitized mice.

【 授权许可】

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