A mannose-binding receptor is expressed on human keratinocytes and mediates killing of Candida albicans

JOURNAL OF INVESTIGATIVE DERMATOLOGY

卷:117

Article

Szolnoky, G ; Bata-Csörgö, Z ; Kenderessy, AS ; Kiss, M ; Pivarcsi, A ; Novák, Z ; Newman, KN ; Michel, G ; Ruzicka, T ; Maródi, L ; Dobozy, A ; Kemény, L

关键词: antimicrobial activity;    Candida infection;    epidermis;    skin;   

DOI  :  10.1046/j.1523-1747.2001.14071.x

来源: Elsevier

【 摘 要 】

Human keratinocytes are known to kill Candida albicans in vitro, but the mechanism of killing is not yet understood. Here, we demonstrate that spontaneous, ultraviolet-B-light-induced, alpha -melanocyte-stimulating-hormone-induced, and interleukin-8-induced Candida killing by keratinocytes can be inhibited with mannan and mannosylated bovine serum albumin (Man-BSA). A polyclonal goat serum raised against the human macrophage mannose receptor stained suprabasal keratinocytes, but no staining was observed on keratinocytes with a monoclonal antibody (mAb(15)) specific for the human macrophage mannose receptor. Mannose-affinity chromatography of keratinocyte extract isolated a 200 kDa protein, and on the Western blot the goat antiserum reacted with a 200 kDa protein. In radioligand binding studies, the binding of I-125-Man-BSA to human keratinocytes was inhibited by mannan in a concentration-dependent manner. Analysis of the binding revealed a single class keratinocyte mannose receptor with a K-D of 1.4 x 10(-8) M and a B-max of 1 x 10(4) binding sites per cell. The binding of I-125-Man-BSA to keratinocytes proved to be time-dependent, acid-precipitable, and Ca2+ - and trypsin-sensitive. After trypsinization the receptors underwent a rapid recovery at 37 degreesC. These results demonstrate the presence of mannose receptor on human keratinocytes, and its active involvement in the killing of Candida albicans.

【 授权许可】

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