Patients with allergic contact dermatitis to nickel and nonallergic individuals display different nickel-specific T cell responses.: Evidence for the presence of effector CD8+ and regulatory CD4+ T cells

JOURNAL OF INVESTIGATIVE DERMATOLOGY

卷:111

Article

Cavani, A ; Mei, D ; Guerra, E ; Corinti, S ; Giani, M ; Pirrotta, L ; Puddu, P ; Girolomoni, G

关键词: allergy;    cytokines;    skin;    T lymphocytes;   

DOI  :  10.1046/j.1523-1747.1998.00334.x

来源: Elsevier

【 摘 要 】

To investigate the mechanisms underlying the expression of allergic contact dermatitis, we compared the characteristics of nickel (Ni)-specific T cell responses in 10 patients with allergic contact dermatitis to Ni and in 10 healthy, nonallergic individuals. CD4(+) T cells purified from peripheral blood of both allergic and nonallergic subjects proliferated similarly to NiSO4 in vitro, with the responses mostly restricted to CD4(+) CD45RO(+) memory T cells. In contrast, Ni-specific CD8(+) T cell responses were detected only in allergic patients. Limiting dilution assay confirmed a high frequency of Ni-specific CD4(+) T cells in both individual categories, and of Ni-specific CD8(+) T cells in allergic patients, but not in nonallergic persons. Ni-specific CD4(+) T cell clones prepared from nonallergic subjects displayed lower interferon-gamma and higher interleukin-10 production compared with T cell clones from allergic patients. The T cell skin-homing receptor, cutaneous lymphocyte-associated antigen, was expressed on the large majority of specific CD4(+) clones from both the groups. Finally, Ni-specific CD8(+) clones prepared from patients also expressed the cutaneous lymphocyte-associated antigen receptor, and released high interferon-gamma and no interleukin-4. In aggregate, the results suggest that the presence of specific CD8(+) T cells and a distinct pattern of cytokine release (e.g., an augmented production of interleukin-10) by CD4(+) T cells can be important elements in determining whether a hapten induces allergy or a silent immune response.

【 授权许可】

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