Interleukin-10 downregulates anti-microbial peptide expression in atopic dermatitis

JOURNAL OF INVESTIGATIVE DERMATOLOGY

卷:125

Article

Howell, MD ; Novak, N ; Bieber, T ; Pastore, S ; Girolomoni, G ; Boguniewicz, M ; Streib, J ; Wong, C ; Gallo, RL ; Leung, DYM

关键词: anti-microbial peptides;    cytokines;    extrinsic atopic dermatitis;    intrinsic atopic dermatitis;   

DOI  :  10.1111/j.0022-202X.2005.23776.x

来源: Elsevier

【 摘 要 】

Recurrent skin infections in extrinsic atopic dermatitis (EAD) may be because of the suppression of anti-microbial peptide (AMP) expression by interleukin (IL)-4 and IL-13. Twenty to thirty percent of AD, however, are classified as intrinsic atopic dermatitis (IAD). They exhibit normal serum IgE levels, no allergen-specific sensitization, and lower levels of IL-4 and IL-13 than EAD. Both forms of AD have increased propensity to skin infection, suggesting a novel mechanism for infection in IAD. In this study, we observed significantly decreased human beta-defensin (HBD)-2 gene expression in the skin of both IAD (p = 0.010) and EAD (p = 0.004), as compared with psoriasis patients. Conversely, IAD (p=0.019) and EAD (p=0.002) skin lesions exhibited elevated IL-10 gene expression when compared with psoriasis. Using primary keratinocytes, we found that the deficiency in AMP expression is an acquired rather than a constitutive defect. Interestingly, neutralizing antibodies to IL-10 augmented the production of tumor necrosis factor-alpha and interferon-gamma by peripheral blood mononuclear cell from AD patients. Additionally, treatment of AD skin explants with anti-IL-10 augmented the expression of both HBD-2 and LL-37. Thus, increased levels of IL-10 may contribute to the AMP deficiency in both IAD and EAD by reducing cytokines that induce AMP.

【 授权许可】

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