Formation of the epidermal calcium gradient coincides with key milestones of barrier ontogenesis in the rodent

JOURNAL OF INVESTIGATIVE DERMATOLOGY

卷:110

Article

Elias, PM ; Nau, P ; Hanley, K ; Cullander, C ; Crumrine, D ; Bench, G ; Sideras-Haddad, E ; Mauro, T ; Williams, ML ; Feingold, KR

关键词: epidermis;    permeability barrier;    protein induced X-ray emission (PIXE);    transepidermal water loss;   

DOI  :  10.1046/j.1523-1747.1998.00151.x

来源: Elsevier

【 摘 要 】

The epidermal permeability barrier forms late in gestation, coincident with decreased lipid synthesis, increased lipid processing, and development of a mature, multilayered stratum corneum. Prior studies have shown that changes in the epidermal Ca++ gradient in vivo regulate lamellar body secretion and lipid synthesis, and modulations in extracellular Ca++ in vitro also regulate keratinocyte differentiation. We asked here whether a Ca++ gradient forms in fetal epidermis in utero, and whether its emergence correlates with key developmental milestones of barrier formation and stratum corneum development. Using either ion precipitation or proton induced X-ray emission analysis of fetal mouse and rat skin, we showed that a Ca++ gradient is not present at gestational days 16-18, prior to barrier formation, and that a gradient forms coincident with the emergence of barrier competence (day 19, mouse; day 20, rat) prior to birth, These results are consistent with a role for Ca++ in the regulation of key metabolic events leading to barrier formation. Whether the calcium gradient is formed actively or passively remains to be determined.

【 授权许可】

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