Expression of the apolipoprotein E gene in the skin is controlled by a unique downstream enhancer

JOURNAL OF INVESTIGATIVE DERMATOLOGY

卷:116

Article

Grehan, S ; Allan, C ; Tse, E ; Walker, D ; Taylor, JM

关键词: apoE mRNA;    enhancer;    epidermis;    hair follicle;    sebaceous gland;    transgenic;   

DOI  :  10.1046/j.1523-1747.2001.00213.x

来源: Elsevier

【 摘 要 】

A distal enhancer that specifies apolipoprotein E gene expression in the skin was identified and characterized by in situ hybridization in transgenic mice generated with constructs of the human apolipoprotein E/C-I/C-IV/C-II gene duster. Transgene constructs containing the enhancer expressed high levels of apolipoprotein E mRNA in the germinative cell layer of the sebaceous gland and in epithelial cells of the hair follicle root sheath. Apolipoprotein E mRNA was also detected in basal epithelial cells of the epidermis. Expression of the human apolipoprotein E transgene at these sites was specified by a unique 1.0 kb enhancer domain located 1.7 kb downstream of the apolipoprotein E gene. No transgene expression was detected in skin epithelial cells in transgenic mice when this enhancer was deleted from the apolipoprotein E gene cluster, The enhancer was used to construct a transgene expression vector that faithfully directed a heterologous cDNA to the normal sites of apolipoprotein E gene expression in epithelial cells of the skin.

【 授权许可】

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