Reduced number and impaired function of circulating γδ T cells in patients with cutaneous primary melanoma

JOURNAL OF INVESTIGATIVE DERMATOLOGY

卷:120

Article

Argentati, K ; Re, F ; Serresi, S ; Tucci, MG ; Bartozzi, B ; Bernardini, G ; Provinciali, M

关键词: fluorescence-activated cell sorter;    human;    melanoma;    tumor immunity;    gamma delta T cells;   

DOI  :  10.1046/j.1523-1747.2003.12141.x

来源: Elsevier

【 摘 要 】

We studied the peripheral representation, in vitro expansion, cytokine production, and cytotoxicity of gammadelta T lymphocytes from 23 patients with cutaneous primary melanoma and 28 healthy subjects. We demonstrated that the absolute number and the percentage of circulating gammadelta(+) T cells were significantly reduced in melanoma patients in comparison with healthy subjects. The decrease was due to a reduction of Vdelta2 T cells, whereas the number of Vdelta1 T cells was not affected. As a consequence, the Vdelta2/Vdelta1 ratio was inverted in melanoma patients. A lower percentage of gammadelta(+) T cells producing tumor necrosis factor-alpha or interferon-gamma was found in melanoma patients. After a 10 d in vitro culture, both the percentage and the expansion index of gammadelta T cells, and in particular of Vdelta2 subset, were significantly reduced in melanoma patients in comparison with healthy subjects. The cytotoxicity of sorted gammadelta T cells against tumor cell lines and the percentage of gammadelta T cells producing perforins were preserved in melanoma patients. The numerical and functional impairment of gammadelta T cells could contribute to the inadequate immune response found in melanoma patients and offers the potentiality for the planning of new approaches of immune therapy of malignant melanoma.

【 授权许可】

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