INVOLVEMENT OF GRANULOCYTES AND THE ADHESION RECEPTORS INTERCELLULAR-ADHESION MOLECULE-1 AND LYMPHOCYTE FUNCTION-ASSOCIATED ANTIGEN-1 IN TISSUE INFLAMMATION-INDUCED BY TH2-TYPE HELPER-CELLS

JOURNAL OF INVESTIGATIVE DERMATOLOGY

卷:104

Article

MULLER, KM ; JAUNIN, F ; MASOUYE, I ; PIGUET, PF ; SAURAT, JH ; HAUSER, C

关键词: POLYMORPHONUCLEAR LEUKOCYTES;    INTERLEUKIN-4;    DELAYED-TYPE HYPERSENSITIVITY;    EXTRAVASATION;    ACTIVATION;   

DOI  :  10.1111/1523-1747.ep12665378

来源: Elsevier

【 摘 要 】

We reported recently that subcutaneously injected, anti-CD3 epsilon-pulsed polyclonal Th2 cells mediate interleukin-4-dependent local tissue inflammation. Because a prominent polymorphonuclear infiltrate was observed in the lesions at the time of maximal tissue swelling, we investigated the involvement of polymorphonuclear leukocytes and their adhesion molecules lymphocyte function-associated antigen-1 (LFA-1) and intercellular adhesion molecule-1 (ICAM-1) in Th2-cell-mediated inflammation. Pretreatment of recipient mice with a depleting monoclonal antibody to neutrophils or with blocking antibodies to LFA-1 or to ICAM-1 completely abrogated tissue swelling in Th2-cell-mediated inflammation. Granulocyte infiltration at 6 h was also inhibited by the antibodies to neutrophils and to ICAM-1, but not by that to LFA-1, Tissue swelling mediated by Th1 cells had different kinetics and was not prevented by administration of anti-neutrophil antibody: maximal edema formation occurred at: 24-48 h, when the predominant cellular infiltrate was mononuclear. Because the Th1-cell-induced infiltrate at 6 h also consisted of granulocytes but was not associated with pronounced edema, the mere presence of infiltrating polymorphonuclear leukocytes seems not to be sufficient to induce edema, Because edema but not granulocyte infiltration was inhibited by anti-LFA-1 and because anti-LFA-1 antibodies are known to inhibit several functions of neutrophils, our results suggest that, in inflammation mediated by Th2 cells, granulocytes induce edema through their activation and/or degranulation.

【 授权许可】

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