A serial analysis of gene expression in sun-damaged human skin

JOURNAL OF INVESTIGATIVE DERMATOLOGY

卷:119

Article

Urschitz, J ; Iobst, S ; Urban, Z ; Granda, C ; Souza, KA ; Lupp, C ; Schilling, K ; Scott, I ; Csiszar, K ; Boyd, CD

关键词: elastic fibers;    epidermis;    full-thickness skin;    photoaging;    SAGE analysis;   

DOI  :  10.1046/j.1523-1747.2002.01829.x

来源: Elsevier

【 摘 要 】

To study the phenotypic changes in human skin associated with repeated sun exposure at the transcription level, we have undertaken a comparative serial analysis of gene expression of sun-damaged preauricular skin and sun-protected postauricular skin as well as sun-protected epidermis. Serial analyses of gene expression libraries, containing multiple mRNA-derived tag recombinants, were made to poly(A(+) )RNA isolated from human postauricular skin and preauricular skin, as well as epidermal nick biopsy samples. 5330 mRNA-derived cDNA tags from the postauricular serial analysis of gene expression library were sequenced and these tag sequences were compared to cDNA sequences identified from 5105 tags analyzed from a preauricular serial analysis of gene expression library. Of the total of 4742 different tags represented in both libraries we found 34 tags with at least a 4-fold difference of tag abundance between the libraries. Among the mRNAs with altered steady-state(1) levels in sun-damaged skin, we detected those encoding keratin 1, macrophage inhibitory factor, and calmodulin-like skin protein. In addition, a comparison of cDNA sequences identified in the serial analysis of gene expression libraries obtained from the epidermal biopsy samples (5257 cDNA tags) and from both full-thickness skin samples indicated that many genes with altered steady-state transcript levels upon sun exposure were expressed in epidermal keratinocytes. These results suggest a major role for the epidermis in the pathomechanism of largely dermal changes in chronically sun-exposed skin.

【 授权许可】

Free