UP-REGULATION OF KERATIN-17 EXPRESSION IN HUMAN HACAT KERATINOCYTES BY INTERFERON-GAMMA

JOURNAL OF INVESTIGATIVE DERMATOLOGY

卷:104

Article

BONNEKOH, B ; HUERKAMP, C ; WEVERS, A ; GEISEL, J ; SEBOK, B ; BANGE, FC ; GREENHALGH, DA ; BOTTGER, EC ; KRIEG, T ; MAHRLE, G

关键词: TYPE II INTERFERON;    IN VITRO;    DIFFERENTIATION;    PSORIASIS;   

DOI  :  10.1111/1523-1747.ep12613492

来源: Elsevier

【 摘 要 】

The immortalized human keratinocyte cell Line HaCaT was used to assess the effect of interferon-gamma (IFN-gamma) on expression of keratin K17. Both IFN-gamma and K17 have been implicated in the pathophysiology of psoriasis. Western and quantitative enzyme-linked immunosorbent assay analyses demonstrated increasing induction of K17 protein by 48 h exposure to IFN-gamma at concentrations of 10, 50, and 250 U/ml. At 50 U/ml IFN-gamma, immunohistochemical analysis revealed numerous K17-positive foci, whereas in situ hybridization demonstrated K17 message in the majority of cells. In addition, at low (5 U/ml) concentrations of IFN-gamma, cell proliferation and protein synthesis decreased, as determined by H-3-thymidine labeling and C-14-amino acid uptake. These data suggest that aberrant K17 expression observed in psoriatic lesions may be a consequence of IFN-gamma overexpression, and that the HaCaT cell line may be a useful in vitro model system to elucidate the underlying mechanisms.

【 授权许可】

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