Water increases the fluidity of intercellular membranes of stratum corneum: Correlation with water permeability, elastic, and electrical resistance properties

JOURNAL OF INVESTIGATIVE DERMATOLOGY

卷:106

Article

Alonso, A ; Meirelles, NC ; Yushmanov, VE ; Tabak, M

关键词: stratum corneum;    ESR;    hydration effect;    membrane fluidity;    permeability;    elasticity;    electrical resistance;   

DOI  :  10.1111/1523-1747.ep12338682

来源: Elsevier

【 摘 要 】

We used the spin label electron spin resonance technique to monitor the hydration effect on the molecular dynamics of lipids at C-5, C-12, and C-16 positions of the allkyl chain. Increase in water content of neonatal rat SC leads to an increase in membrane fluidity, especially in the region near the membrane-water interface. The effect is less pronounced deeper inside the hydrophobic core. The reorientational correlation time at the C-16 position of hydrocarbon chains showed a higher change up to similar to 18% (w/w) of water content. This behavior was accompanied by an exponential decay both in elastic modulus and electrical resistance with water content. On the contrary, the segmental motion at C-5 and C-12 positions of the chain and the permeability constant increased in the range of around 18% (w/w) up to the fully hydrated condition (58 +/- 7%). Our results give a better characterization of the fluidity of SC and show that it is the principal parameter involved in the mechanism of the permeability of different compounds through skin.

【 授权许可】

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