High-performance, two-step/Bi-enzyme lactate biofuel cell with lactate oxidase and pyruvate oxidase

JOURNAL OF POWER SOURCES

卷:498

Article

Shitanda, Isao^1,2  Hirano, Kai¹  Loew, Noya¹  Watanabe, Hikari¹  Itagaki, Masayuki^1,2  Mikawa, Tsutomu³ 

[1] Tokyo Univ Sci, Fac Sci & Technol, Dept Pure & Appl Chem, 2641 Yamazaki, Noda, Chiba 2788510, Japan

[2] Tokyo Univ Sci, Res Inst Sci & Technol, 2641 Yamazaki, Noda, Chiba 2788510, Japan

[3] RIKEN Ctr Biosyst Dynam Res, Tsurumi Ku, 1-7-22 Suehiro Cho, Yokohama, Kanagawa 2300045, Japan

关键词: Biofuel cell;    Lactate oxidase;    Pyruvate oxidase;    Two-step oxidation;    Two-enzyme bioanode;   

DOI  :  10.1016/j.jpowsour.2021.229935

来源: Elsevier

【 摘 要 】

The performance of biofuel cells (BFCs) depends on the number of electrons that can be harvested from one fuel molecule by the bioanode, as well as many other factors. Regular, one-enzyme bioanodes can usually harvest two electrons per fuel molecule, lowering the conversion efficiency of chemical energy stored in the fuel to electrical energy. In this study, we successfully develop a high-performance BFC with a two-enzyme bioanode that can harvest four electrons by oxidizing lactate to acetylphosphate through a two-step enzymatic reaction that uses lactate oxidase (LOx) and pyruvate oxidase (POx). A current of 1 mA is obtained from the BFC for 2500 s, with only a slight decrease in the cell voltage, reaching 0 V at 3010 s. During this time, a total of 1253 mJ is harvested. This is approximately 1.8-fold the time and double the energy that is obtained from a corresponding one-enzymebioanode BFC employing only LOx, thus confirming that twice the number of electrons are harvested per lactate molecule.

【 授权许可】

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