In vitro comparative analysis of human dental stem cells from a single donor and its neuronal differentiation potential evaluated by electrophysiology

LIFE SCIENCES

卷:154

Article

Ullah, Imran¹  Subbarao, Raghavendra Baregundi¹  Kim, Eun-Jin^2,3  Bharti, Dinesh¹  Jang, Si-Jung¹  Park, Ji-Sung¹  Shivakumar, Sharath Belame¹  Lee, Sung-Lim¹  Kang, Dawon^2,3  Byun, June-Ho⁴  Park, Bong-Wook⁴  Rho, Gyu-Jin¹ 

[1] Gyeongsang Natl Univ, Dept Theriogenol & Biotechnol, Coll Vet Med, Jinju 660701, South Korea

[2] Gyeongsang Natl Univ, Sch Med, Dept Physiol, Jinju 660751, South Korea

[3] Gyeongsang Natl Univ, Sch Med, Inst Hlth Sci, Jinju 660751, South Korea

[4] Gyeongsang Natl Univ, Inst Hlth Sci, Sch Med, Dept Oral & Maxillofacial Surg, Jinju 660701, South Korea

关键词: Dental tissues;    MSCs;    Neuronal differentiation;    Electrophysiology;    Synaptic markers;   

DOI  :  10.1016/j.lfs.2016.04.026

来源: Elsevier

【 摘 要 】

Aims: The aim of this study was to find out a mesenchymal stem cells(MSCs) source from human dental tissues of the same donor (follicle, papilla and pulp), which exhibits higher neurogenic differentiation potential in vitro. Main methods: MSCs were isolated from dental tissues (follicle, papilla and pulp) by digestion method. All MSCs were analyzed for pluripotent makers by western blot, cell surface markers by flow cytometry, adipo- and osteocytes markers by RT-qPCR. The neuronal differentiated MSCs were characterized for neuronal specific markers by RT-qPCR and immunofluorescence. Functional neuronal properties were analyzed by electrophysiology and synaptic markers expression. Key findings: All MSCs expressed pluripotent markers (Oct4, Sox2 and Nanog) and were found positive for mesenymal markers (CD44, CD90, CD105) while negative for hematopoietic markers (CD34 and CD45). Furthermore, MSCs were successfully differentiated into adipocytes, osteocytes and trans-differentiated into neuronal cells. Among them, dental pulp derived MSCs exhibits higher neurogenic differentiation potential, in term of expression of neuronal specific markers at both gene and protein level, and having higher Na+ and K+ current with the expression of synaptic markers. Significance: The three types of dental MSCs from a single donor broadly possessed similar cellular properties and can differentiate into neuronal cells; however, pulp derived MSCs showed higher neurogenic potential than the follicle and papilla, suggesting their use in future stem cells therapy for the treatment of neurodegenerative disorders. (C) 2016 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

【 授权许可】

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