被撤回的出版物: Ginsenoside Rg1 defenses PC-12 cells against hydrogen peroxide-caused damage via up-regulation of miR-216a-5p (Retracted article. See vol. 305, 2022)

LIFE SCIENCES

卷:236

Article; Retracted Publication

Yi, Guangkun¹  Liu, Li²  Lv, Chaoliang¹  Wei, Yanchun¹  Yan, Tingzhen¹ 

[1] Jining 1 Peoples Hosp, Dept Spine Surg, 6 Jiankang Rd, Jining 272011, Shandong, Peoples R China

[2] Jining 1 Peoples Hosp, Dept Cardiol, Jining 272011, Shandong, Peoples R China

关键词: Ginsenoside Rg1;    miR-216a-5p;    Cell injury;   

DOI  :  10.1016/j.lfs.2019.116948

来源: Elsevier

【 摘 要 】

Background: Spinal cord injury (SCI) is a destructive trauma accompanied with local injury. Ginsenoside Rg1 exerts anti-apoptosis and anti-autophagy properties. Our goal was to study the protective mechanism of Rg1 in attenuating cell injury. Methods: MiR-216a-5p inhibitor was transfected into PC-12 cells to verify the growth promoting roles of miR-216a-5p, then cells were pre-treated by Rg1 for 24 h and treated by 300 mu M hydrogen peroxide (H2O2) for 1 h. Cell viability and apoptosis were tested through Cell Counting Kit-8 (CCK-8) and flow cytometry, respectively. Expression of miR-216a-5p and cell damage relative factors was tested via qRT-PCR and Western blot experiments. Results: H2O2 induced cell activity suppression, apoptosis and clear autophagy well at the concentration of 300 mu M Rg1 attenuated H2O2-induced cell injury at the concentration of 200 mu M that it elevated cell activity, attenuated apoptosis and autophagy and activated phosphatidylinositol 3 kinase (PI3K)/AMP-activated protein kinase (AKT) and AMP-activated protein kinase (AMPK) signal pathways. Further, miR-216a-5p was up-regulated by Rg1. Conclusion: Our study demonstrated that Rg1 attenuated H2O2-caused cell injury through positively regulated miR-216a-5p.

【 授权许可】

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