Monohydroxylated polycyclic aromatic hydrocarbons inhibit both osteoclastic and osteoblastic activities in teleost scales

LIFE SCIENCES

卷:84

Article

Suzuki, Nobuo¹  Hayakawa, Kazuichi²  Kameda, Takayuki²  Triba, Akira²  Tang, Ning²  Tabata, Makoto J.³  Takada, Koji⁴  Wada, Shigehito⁵  Omori, Katsunori⁶  Srivastav, Ajai K.⁷  Mishima, Hiroyuki⁸  Hattori, Atsuhiko⁹ 

[1] Kanazawa Univ, Inst Nat & Environm Technol, Noto Marine Lab, Noto, Ishikawa 9270553, Japan

[2] Kanazawa Univ, Inst Med Pharmaceut & Hlth Sci, Kanazawa, Ishikawa 9201192, Japan

[3] Tokyo Med & Dent Univ, Grad Sch, Sect Biostruct Sci, Bunkyo Ku, Tokyo 1138549, Japan

[4] Jikei Univ, Sch Med, Dept Biochem, Minato Ku, Tokyo 1058461, Japan

[5] Toyama Univ, Fac Med, Dept Oral & Maxillofacial Surg, Toyama 9300194, Japan

[6] Japan Aerosp Explorat Agcy, Tsukuba, Ibaraki 3058505, Japan

[7] Gorakhpur Univ, Dept Zool, Gorakhpur 273009, Uttar Pradesh, India

[8] Kochi Gakuen Coll, Kochi 7800955, Japan

[9] Tokyo Med & Dent Univ, Coll Liberal Arts & Sci, Dept Biol, Chiba 2720827, Japan

关键词: Monohydroxylated polycyclic aromatic hydrocarbons;    Teleost scale;    Osteoclasts;    Osteoblasts;    Bone metabolism;    Cathepsin K;    Insulin-like growth factor-I;   

DOI  :  10.1016/j.lfs.2009.01.008

来源: Elsevier

【 摘 要 】

Aims: We previously demonstrated that monohydroxylated polycyclic aromatic hydrocarbons (OHPAHs) bound to a human estrogen receptor (ER) by a yeast two-hybrid assay, but polycyclic aromatic hydrocarbons did not have a binding activity. Therefore, the direct effect of 3-hydroxybenz[a]anthracene (3-OHBaA) and 4-hydroxybenz[a]anthracene (4-OHBaA) on osteoclasts and osteoblasts in teleosts was examined. As a negative control, 1 -hydroxypyrene (1-OHPy), which has no binding activity to human ER, was used. Main methods: The effect of CHPAHs on osteoclasts and osteoblasts was examined by an assay system using teleost scale as each marker: tartrate-resistant acid phosphatase for osteoclasts and alkaline phosphatase for osteoblasts. Changes in cathepsin K (an osteoclastic marker) and insulin-like growth factor-1 (IGF-1) (an osteoblastic marker) mRNA expressions in 4-OHBaA-treated goldfish scales were examined by using a reverse transcription-polymerase chain reaction. Key findings: In both goldfish (a freshwater teleost) and wrasse (a marine teleost), the osteoclastic activity in the scales was significantly suppressed by 3-OHBaA and 4-OHBaA, although 1-OHPy did not affect the osteoclastic activity. In reference to osteoblasts, the osteoblastic activity decreased with both 3-CHBaA and 4-CHBaA and did not change with the 1-OHPy treatment. However, 17 beta-estradiol (E(2)) significantly increased both the osteoclastic and osteoblastic activities in the scales of both goldfish and wrasse. The mRNA expressions of both cathepsin K and IGF-1 decreased in the 4-OHBaA-treated scales but increased in the E(2)-treated scales. Significance: The current data are the first to demonstrate that 3-OHBaA and 4-OHBaA inhibited both osteoclasts and osteoblasts and disrupted the bone metabolism in teleosts. (C) 2009 Elsevier Inc. All rights reserved.

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