Acute exercise alters homocysteine plasma concentration in an intensity-dependent manner due increased methyl flux in liver of rats

LIFE SCIENCES

卷:196

Article

Riberio, Diogo Farias¹  Cella, Paola Sanchez¹  Costa Mendes da Silva, Lilian Eslaine²  Jordao, Alceu Afonso²  Deminice, Rafael¹ 

[1] Univ Estadual Londrina, Dept Phys Educ, Rodovia Celso Garcia Cid,Pr 445 Km 380, Londrina, Parana, Brazil

[2] Univ Sao Paulo, Fac Med Ribeirao Preto, Nutr & Metab, Sao Paulo, Brazil

关键词: Homocysteine;    Acute exercise;    Methionine;   

DOI  :  10.1016/j.lfs.2018.01.003

来源: Elsevier

【 摘 要 】

Purpose: We aimed to determine the effects of different intensities of acute exercise on Hcy plasma levels, and the exercise-induced changes in Hcy liver metabolism. Method: First, thirty-two Wistar rats were randomly submitted to an acute bout of swimming exercise carrying a load of 2% (n=8), 4% (n=8) and 6% (n=8) of their total body weight attached in their tail. Control rats remained rested (n=8). Blood samples were taken from tail vein for plasma S-containing amino acids determination before (Rest) and post, 1, 2, 3, 4, 6, and 10 h after acute swimming exercise. Second, 56 exercised rats (4% loads) were euthanized before (Rest) and1, 2, 3, 4, 6, and 10 h after acute swimming exercise. Blood and liver samples were collected for amino acids and keys genes involved in the Hcy metabolism assay. Results: Acute exercise increases (P < 0.05) plasma Hcy concentration in an intensity-dependent manner (rest 7.7 +/- 0.8; 6% load 13.8 +/- 3.6; 4% load 12.2 +/- 2.9 +/- and 2% load 10.1 +/- 2.6, mu mol/L); this increase is transient and does not promote hyperhomocysteinemia (< 15 mu mol/L). Exercise-induced increased plasma Hcy was accompanied by the decreased liver S-adenosylmethionine/S-adenosylhomocysteine ratio and elevated MAT1a mRNA content. Acute exercise also caused elevated mRNA of key enzymes of transsulfuration (CBS) and remethylation (BHMT and the MTRR). Conclusion: Our data provided evidence that acute exercise increases plasma Hcy concentration due to the augmented requirement for methylated compounds that increases liver SAM consumption. Also, Hcy remethylation and transsulfuration are coordinately regulated to maintain methyl balance.

【 授权许可】

Free