High-fat diet and caffeine interact to modulate bone microstructure and biomechanics in mice

LIFE SCIENCES

卷:276

Article

de Souza, Fernanda Batista¹  Novaes, Romulo Dias²  Ferreira Santos, Cynthia Fernandes³  de Deus, Franciele Angelo⁴  Santos, Felipe Couto⁵  Maia Ladeira, Luiz Carlos⁵  Goncalves, Reggiani Vilela⁵  Sena Bastos, Daniel Silva⁵  Ferreira Souza, Ana Claudia⁶  Machado-Neves, Mariana⁵  dos Santos, Eliziaria Cardoso^1,3 

[1] Fed Univ Jequitinhonha & Mucuri Valleys, Postgrad Program Rehabil & Funct Performance, Diamantina, MG, Brazil

[2] Univ Fed Alfenas, Inst Biomed Sci, Dept Struct Biol, Alfenas, MG, Brazil

[3] Fed Univ Jequitinhonha & Mucuri Valleys, Sch Med, Campus JK,MGT 367 Km 583,5000 Alto da Jacuba, BR-39100000 Diamantina, MG, Brazil

[4] Fed Univ Jequitinhonha & Mucuri Valleys, Dept Physiotherapy, Diamantina, MG, Brazil

[5] Univ Fed Vicosa, Dept Gen Biol, Vicosa, MG, Brazil

[6] Univ Fed Rural Rio de Janeiro, Dept Anim Biol, Seropedica, RJ, Brazil

关键词: Bone morphology;    Caffeine;    Cafeteria diet;    Tissue remodeling;    Bone health;   

DOI  :  10.1016/j.lfs.2021.119450

来源: Elsevier

【 摘 要 】

Aims: Although excessive fat and caffeine intake are independent risk factors for bone microstructural and functional disturbances, their association remains overlooked. Thus, we investigated the impact of high-fat diet (HFD) and caffeine alone and combined on serum lipid profile, bone microstructure, micromineral distribution and biomechanical properties. Methods: Forty female C57BL/6 mice were randomized into 4 groups daily treated for seventeen weeks with standard diet (SD) or HFD (cafeteria diet) alone or combined with 50 mg/kg caffeine. Key findings: The association between HFD and caffeine reduced the weight gain compared to animals receiving HFD alone. Caffeine alone or combined with HFD increases total and HDL cholesterol circulating levels. HFD also reduced calcium, phosphorus and magnesium bone levels compared to the groups receiving SD, and this reduction was aggravated by caffeine coadministration. From biomechanical assays, HFD combined with caffeine increased bending strength and stiffness of tibia, a finding aligned with the marked microstructural remodeling of the cortical and cancellous bone in animals receiving this combination. Significance: Our findings indicated that HFD and caffeine interact to induce metabolic changes and bone microstructural remodeling, which are potentially related to bone biomechanical adaptations in response to HFD and caffeine coadministration.

【 授权许可】

Free