Bladder overactivity induced by psychological stress in female mice is associated with enhanced bladder contractility

LIFE SCIENCES

卷:265

Article

West, Eliza G.¹  Sellers, Donna J.¹  Chess-Williams, Russ¹  McDermott, Catherine¹ 

[1] Bond Univ, Fac Hlth Sci & Med, Ctr Urol Res, Robina, Qld 4229, Australia

关键词: Psychological stress;    Bladder;    Water avoidance stress;    Urinary frequency;   

DOI  :  10.1016/j.lfs.2020.118735

来源: Elsevier

【 摘 要 】

Aims: To investigates the effects of water avoidance stress on voiding behaviour and functional bladder responses in mice. Main methods: Mice in the Stress group were exposed to water avoidance stress (WAS) for 1 h/day for 10 days, Controls were age-matched and housed normally. Voiding behaviour was measured periodically throughout the stress protocol and bladders were isolated 24-h after final stress exposure to measure bladder compliance, spontaneous phasic activity, contractile responses, and release of urothelial mediators. Key findings: Repeated stress exposure induced a significant increase in plasma corticosterone levels in the WAS group compared to control. An overactive bladder phenotype was observed in WAS mice, causing a significant increase in the number of voiding events observed from as early as day-3, and a 7-fold increase following 10-days' stress. This increase in voiding frequency was associated with a significant decrease in void size, an increase in the number of small voids, but no change in total voided volume. Bladders from stressed mice showed a significant increase in the maximum responses to the muscarinic agonist carbachol (p < 0.01), in addition to enhanced pressure responses to the purinergic agonists ATP (p < 0.05) and alpha beta-mATP (p < 0.05), and non-receptor mediated contractions to KCl (p < 0.05) compared to controls. Nerve-mediated bladder contractions to electric field stimulation were not significantly affected by stress, nor were spontaneous phasic contractions or release of urothelial ATP and acetylcholine. Significance: Repeated exposure to water avoidance stress produced an overactive bladder phenotype, confirmed by increased voiding frequency, and associated with enhanced bladder contractile responses.

【 授权许可】

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