Oral supplementation of melatonin protects against lupus nephritis renal injury in a pristane-induced lupus mouse model

LIFE SCIENCES

卷:193

Article

dos Santos, Mariane^1,2,3  Favero, Gaia⁴  Bonomini, Francesca^4,5  Stacchiotti, Alessandra^4,5  Rodella, Luigi Fabrizio^4,5  Veronese, Francisco Verissimo^1,2,3  Rezzani, Rita^4,5 

[1] Univ Fed Rio Grande do Sul, Grad Program Med Sci, Porto Alegre, RS, Brazil

[2] Hosp Clin Porto Alegre, Div Nephrol, Porto Alegre, RS, Brazil

[3] Hosp Clin Porto Alegre, Expt Res Ctr, Lab Mol Biol Appl Nephrol, Porto Alegre, RS, Brazil

[4] Univ Brescia, Dept Clin & Expt Sci, Anat & Physiopathol Div, Viale Europa 11, I-25123 Brescia, Italy

[5] Univ Brescia, Interdipartimental Univ Ctr Res Adapt & Regenerat, Brescia, Italy

关键词: Apoptosis;    Fibrosis;    Lupus;    Mesangial cells;    Oxidative stress;    Proximal tubule;   

DOI  :  10.1016/j.lfs.2017.10.038

来源: Elsevier

【 摘 要 】

Aims: Since lupus nephritis (LN) etiopathogenesis is not fully understood, herein we investigated the morphological basis of LN in mice induced with pristane. Main methods: To evaluate the melatonin effects in these animals, we studied the renal cytoarchitecture by means of morphological analyses, immunofluorescence expression of specific markers related to fibrosis, oxidative stress, inflammation and apoptosis. Key findings: We observed that pristane-LN mice have serious alterations in the kidney cytoarchitecture, i.e. tubular degeneration, glomerular hypercellularity, matrix mesangial expansion and interstitial inflammation. The pristane-induced LN mice treated with melatonin exhibited a well preserved cytoarchitecture. Significance: Our results document that LN etiopathogenesis is related to both tubular damage and glomerular lesions. We suggest that it is essential to take in consideration both these lesions for LN diagnosis and classification. Clearly, we show that the use of melatonin may be a possible therapeutic strategy for improvement the renal injury in this disorder.

【 授权许可】

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