L-NAME-treatment alters ectonucleotidase activities in kidney membranes of rats

LIFE SCIENCES

卷:87

Article

Furstenau, Cristina Ribas¹  Ramos, Denise Barbosa¹  Vuaden, Fernanda Cenci¹  Casali, Emerson Andre²  Monteiro, Priscilla de Souza³  Trentin, Danielle da Silva¹  Gossenheimer, Agnes Nogueira¹  Bogo, Mauricio Reis⁴  Bonan, Carla Denise⁵  Morais Barreto-Chaves, Maria Luiza³  Freitas Sarkis, Joao Jose¹  Wofchuk, Susana Tchernin¹ 

[1] Univ Fed Rio Grande do Sul, Inst Ciencias Basicas Saude, Dept Bioquim, BR-90035003 Porto Alegre, RS, Brazil

[2] IPA, Ctr Univ Metodista Sul, Porto Alegre, RS, Brazil

[3] Univ Sao Paulo, Lab Biol Celular & Anat Func, Dept Anat, Inst Ciencias Biomed, Sao Paulo, Brazil

[4] Pontificia Univ Catolica Rio Grande do Sul, Fac Biociencias, Lab Biol Genom & Mol, Porto Alegre, RS, Brazil

[5] Pontificia Univ Catolica Rio Grande do Sul, Fac Biociencias, Lab Neuroquim & Psicofarmacol, Porto Alegre, RS, Brazil

关键词: Ectonucleotidase;    L-NAME;    Hypertension;    ATP;    Adenosine;    Kidney;    Rat;   

DOI  :  10.1016/j.lfs.2010.07.008

来源: Elsevier

【 摘 要 】

Aims: To investigate the effect of N omega-Nitro-L-arginine methyl ester CL-NAME) treatment, known to induce a sustained elevation of blood pressure, on ectonucleotidase activities in kidney membranes of rats. Main methods: L-NAME (30 mg/kg/day) was administered to Wistar rats for 14 days in the drinking water. Enzyme activities were determined colorimetrically and their gene expression patterns were analyzed by semi-quantitative RT-PCR. The metabolism of ATP and the accumulation of adenosine were evaluated by HPLC in kidney membranes from control and hypertensive rats. PKC phosphorylation state was investigated by Western blot. Key findings: We observed an increase in systolic blood pressure from 115 +/- 12 mmHg (control group) to 152 18 mmHg (L-NAME-treated group). Furthermore, the hydrolysis of ATP, ADP, AMP, and p-Nph-5'TMP was also increased (17%, 35%, 27%, 20%, respectively) as was the gene expression of NTPDase2, NTPDase3 and NPP3 in kidneys of hypertensive animals. Phospho-PKC was increased in hypertensive rats. Significance: The general increase in ATP hydrolysis and in ecto-5'-nucleotidase activity suggests a rise in renal adenosine levels and in renal autoregulatory responses in order to protect the kidney against the threat presented by hypertension. (C) 2010 Elsevier Inc. All rights reserved.

【 授权许可】

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