Effects of fixatives on histomagnetic evaluation of iron in rodent spleen

JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS

卷:521

Article

Walsh, Kevin J.¹  Shah, Stavan, V²  Wei, Ping³  Oberdick, Samuel D.⁴  Dickson-Karn, Nicole M.⁵  McTigue, Dana M.³  Agarwal, Gunjan^1,6 

[1] Ohio State Univ, Biophys Program, Columbus, OH 43210 USA

[2] Ohio State Univ, Dept Biomed Engn, Columbus, OH 43210 USA

[3] Ohio State Univ, Dept Neurosci, Columbus, OH 43210 USA

[4] Univ Colorado, Dept Phys, Boulder, CO 80309 USA

[5] Ohio State Univ, Dept Chem & Biochem, Columbus, OH 43210 USA

[6] Ohio State Univ, Dept Mech & Areospace Engn, Columbus, OH 43210 USA

关键词: Histology;    Magnetometry;    Magnetic force microscopy;    Ferritin;    Iron;   

DOI  :  10.1016/j.jmmm.2020.167531

来源: Elsevier

【 摘 要 】

Characterizing the iron distribution in tissue sections is important for several pathologies. Iron content in excised tissue is typically analyzed via histochemical stains, which are dependent on sample preparation and staining protocols. In our recent studies, we examined how magnetic properties of iron can also be exploited to characterize iron distribution in tissue sections in a label free manner. To enable a histomagnetic characterization of iron in a wide variety of available tissues, it is important to extend it to samples routinely prepared for histochemical staining, which often involve use of chemical fixatives. In this study, we took a systematic approach to determine differences between unfixed and formalin-fixed murine spleen tissues in histomagnetic characterization of iron. Superconducting quantum interference device (SQUID) magnetometry and magnetic force microscopy (MFM) were used for macro- and micro-scale histomagnetic characterization. Perl's stain was used for histochemical characterization of ferric (Fe3+) iron on adjacent sections as that used for MFM analysis. While histochemical analysis revealed a substantial difference in the dispersion of the stain between fixed versus unfixed samples, histomagnetic characterization was not dependent on chemical fixation of tissue. The results from this study reveal that histomagnetic characterization of iron is free from staining artifacts which can be present in histochemical analysis.

【 授权许可】

Free