Application of magnetic carriers to two examples of quantitative cell analysis

JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS

卷:427

Article

Zhou, Chen¹  Qian, Zhixi¹  Choi, Young Suk¹  David, Allan E.¹  Todd, Paul²  Hanley, Thomas R.¹ 

[1] Auburn Univ, Dept Chem Engn, 212 Ross Hall, Auburn, AL 36849 USA

[2] Techshot Inc, 7200 Highway 150, Greenville, IN 47124 USA

关键词: Magnetophoretic mobility;    Nanoparticle uptake;    Cell separation;    Cell velocimeter;   

DOI  :  10.1016/j.jmmm.2016.11.009

来源: Elsevier

【 摘 要 】

The use of magnetophoretic mobility as a surrogate for fluorescence intensity in quantitative cell analysis was investigated. The objectives of quantitative fluorescence flow cytometry include establishing a level of labeling for the setting of parameters in fluorescence activated cell sorters (FACS) and the determination of levels of uptake of fluorescently labeled substrates by living cells. Likewise, the objectives of quantitative magnetic cytometry include establishing a level of labeling for the setting of parameters in flowing magnetic cell sorters and the determination of levels of uptake of magnetically labeled substrates by living cells. The magnetic counterpart to fluorescence intensity is magnetophoretic mobility, defined as the velocity imparted to a suspended cell per unit of magnetic ponderomotive force. A commercial velocimeter available for making this measurement was used to demonstrate both applications. Cultured Gallus lymphoma cells were immunolabeled with commercial magnetic beads and shown to have adequate magnetophoretic mobility to be separated by a novel flowing magnetic separator. Phagocytosis of starch nanoparticles having magnetic cores by cultured Chinese hamster ovary cells, a CHO line, was quantified on the basis of magnetophoretic mobility.

【 授权许可】

Free