SANS contrast variation study of magnetoferritin structure at various iron loading

JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS

卷:377

Article

Melnikova, Lucia¹  Petrenko, Viktor I.^2,3  Avdeev, Mikhail V.²  Ivankov, Oleksandr I.^2,3,4  Bulavin, Leonid A.³  Garamus, Vasil M.⁵  Almasy, Laszlo⁶  Mitroova, Zuzana¹  Kopcansky, Peter¹ 

[1] SAS, Inst Expt Phys, Kosice 04001, Slovakia

[2] Joint Inst Nucl Res, Moscow 141980, Russia

[3] Kyiv Tanis Slievchenko Natl Univ, UA-01033 Kiev, Ukraine

[4] Moscow Inst Phys & Technol, Lab Adv Studies Membrane Prot, Dolgoprudnyi 141700, Russia

[5] Helmholtz Zentrum Geesthacht, Ctr Mat & Coastal Res, D-21502 Geesthacht, Germany

[6] HAS, Wiper Res Ctr Phys, H-1525 Budapest, Hungary

关键词: SANS contrast variation method;    Magnetoferritin;    Iron oxides;    Apoferritin;   

DOI  :  10.1016/j.jmmm.2014.10.085

来源: Elsevier

【 摘 要 】

Magnetoferritin, a synthetic derivate of iron storage protein - ferritin, has been synthesized with different iron oxide loading values. Small-angle neutron scattering experiments were applied to study the structure of magnetoferritin solutions using contrast variation method by varying the light to heavy water ratio of the solvent. Higher iron loading leads to increase of the neutron scattering length density of magnetoferritin and also to the increase of the polydispersity of complexes. The formation of the magnetic core and the variation of the protein shell structure upon iron loading are concluded. (C) 2014 Elsevier B.V. All rights reserved.

【 授权许可】

Free